耳穴贴压对睡眠剥夺大鼠tlr4信号通路关键基因mrna表达影响-effect of auricular point pressing on mrna expression of key genes in tl r4 signaling pathway in sleep deprivation rats.docxVIP

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耳穴贴压对睡眠剥夺大鼠tlr4信号通路关键基因mrna表达影响-effect of auricular point pressing on mrna expression of key genes in tl r4 signaling pathway in sleep deprivation rats.docx

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耳穴贴压对睡眠剥夺大鼠tlr4信号通路关键基因mrna表达影响-effect of auricular point pressing on mrna expression of key genes in tl r4 signaling pathway in sleep deprivation rats

ABSTRACTObjective: 1. To observe the effect of auricular-plaster therapy on the sleep duration of (nembutal) sleep-deprived rats.To observe the effect of auricular-plaster therapy on both the visceral index and the contents of mRNA in key genes, like TLR4, MyD88, IRAK1, TRAF6, NF-κB, TRIF, etc., of PCPA sleep-deprived rats.To probe into the mechanism of treating insomnia with auricular-plaster therapy and the relationship between auricular-plaster therapy and the immune system.Methods: 1. Observe changes in the rats’ sleep duration among control group, model group and auricular-plaster group by measuring the sleep duration of (nembutal) sleep-deprived rats.2. Divide the rats randomly into four groups: control group, model group, Diazepam group and auricular-plaster group. The rats will be deprived of sleep by means of PCPA intraperitoneal injection, and then be treated with auricular-plaster therapy for 5 days. After 5 days, changes in splenic and thymic index will be detected by PCR to check the content of key gene mRNA in TLR4 signaling pathway.Results:1. The sleep duration of model and auricular-plaster group lasts longer than that of control group, which manifests a significant difference (P＜0.05); After being treated with auricular-plaster, the sleep-deprived rats in auricular-plaster group sleep apparently less thanthose in model group, which manifests a significant difference (P＜0.05).Sleep deprivation by means of PCPA injection works out.The increased body weight of model group is lower than that of control group, auricular-plaster group and Diazepam group,which manifests a significant difference (P＜0.05).The thymus visceral index of model group is lower than that of control group, auricular-plaster group and Diazepam group, which manifests a significant difference (P＜0.05).5.After rats in model group being deprived of sleep, expression of the key gene mRNA, like TLR4、IRAK1、 TRAF6、NF-κB、TRIF etc., doubles than that of control group. And it drops apparently t