番茄ant基因家族的鉴定及slail1-2基因的功能研究-identification of tomato ant gene family and functional study of sla il 1 - 2 gene.docxVIP

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番茄ant基因家族的鉴定及slail1-2基因的功能研究-identification of tomato ant gene family and functional study of sla il 1 - 2 gene.docx

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番茄ant基因家族的鉴定及slail1-2基因的功能研究-identification of tomato ant gene family and functional study of sla il 1 - 2 gene

ABSTRACTANT (AINTEGUMENTA) transcription factors family is a kind of DNA binding protein, which is unique in the plant kingdom and is able to regulate gene expression at mRNA transcription level. ANT gene family is the member of the AP2 subfamily witch is in the AP2/ERF family of transcription factors in plant. AP2 family members included 2 AP2/ERF domains involves extensively in regulation of plant organs development. Tomato (Solanum lycopersicum) is one of the important worldwide planting, processing materials, the most important representatives of solanaceae crops, and also the most important model plant for the studies on fleshy fruit development and maturation. However, there is little research on ANT gene family in tomato.We also constructed the SlAIL1-2 expression vector, antisense inhibition vector and GFP fusion expression vector to study the subcellular localization of SlAIL1-2 and its responds to hormones. For further search, we analyzed the expression patterns of different parts in floral organ and in different period of pistil by Quantitative PCR. We got several SlAIL1-2 over expression and antisense inhibition transgenic plants, observed the T0 generation plants? phenotype and detected the expression of related genes. Then, we can preliminary supposed that SlAIL1-2 plays an important role in the process of tomato development. The main experimental results are as follows:① In order to identify tomato ANT family members, we used Arabidopsis aminoacid sequences as reference sequences to did the homologous retrieval through Tomato genome database. We identified 5 members which located in the tomato chromosome 4, 5, 7, 2 and 11. The amino acid length they encoded is between 464aa (SlAIL7) ~ 654aa (SlAIL1-2), their molecular weight size is between 50.70 KD ~ 72.00 KD, theoretical isoelectric point is between 6.03 (SlAIL7) ~ 8.54 (SlAIL5), and they all have 7 or 8 introns.② In the structure analysis of tomato ANT family, we found that these fivemembers all in