仿刺参纵肌带的再生及相关基因表达研究-study on the regeneration of the longitudinal muscle band of stichopus japonicus and the expression of related genes.docxVIP

Ab Abstract PAGE PAGE VI Abstract Apostichopus japonicus under adverse environmental conditions, such as water pollution, high water temperature, too dense or certain strong stimulation, will lead to body contraction, cloaca fracture and discharge some or all of the internal organs. This is a self-preservation measure against enemy and stress environment of Apostichopus japonicus.The Apostichopus japonicus have strong ability of regeneration, that won’t die after remove some of the organs from the body, and can regenerate complete new organs. Because of its organ regeneration and repair ability and its important position on evolution on the classification of organisms, study the regeneration mechanism of Apostichopus japonicus has high scientific research value, and has important significance to the higher organisms’ cell proliferation, differentiation and tissue repair mechanism. Using Apostichopus japonicus as test materials to refer to human surgical technique of suture, attempt to suture the surgical site, and established a kind of surgical suture method on sea cucumber body wall. The results obtained the ideal effect, effectively solved the death problem caused by the wound which is difficult to heal. Apostichopus japonicus were put into 0.54 mol/L magnesium sulfate to narcotize for 1h in dissecting tray, then about a 2cm incision along the abdomen distance anal 1/3 on longitudinal, deep to the body cavity which was cut by sterilizated scalpel. Then Apostichopus japonicus were put back and well cultivated in the seawater with antibiotics after suture. The results showed that the wounds of experimental group basicly grew together at 10d, and there was no distinction with normal tissue at 20d. In the experimental group, the skin ulceration rate is 10% and mortality is only 5%. However,in control group(without suture)，the time of wound healing delayed 10 days with the test group on average. In addition, the skin ulceration rate reaches as high as 58.7% and the