仿生型分子信号对烟草生物碱的抑制及其可能机制的分析-inhibitory effect of biomimetic molecular signals on tobacco alkaloids and its possible mechanism analysis.docxVIP

leaves, especially in the upper leaves, was much higher than that of roots and stalks after topping. Whether before topping or after topping, the total alkaloids content of stalks was lowest in the whole growth process of tobacco plants. The total alkaloids content fell by an average of more than 20% when BSM was employed to tobacco plants after topping. The effect of BSM to upper leaves of flue-cured tobacco was obviously higher than that of middle leaves, but the result of BSM to upper leaves of burley tobacco was obviously lower than that of middle leaves. Reduction rate of BSM to roots and stems differed according to various growth processes, which peaked at the fifth day as for flue-cured tobacco and the fifteenth day as for burley tobacco. In general, the fall rate of total alkaloids after using BSM manifested leavestootsstems.3. The activity of lipoxygenase (LOX) and ornithine decarboxylase (ODC) of different treatment tobacco plants were determined so as to revel the possible mechanism that BSM could lower the tobacco alkaloids content of tobacco plants after topping. The LOX variation trend of topping and applying BSM resembled that of mere topping; both of them initially raised gradually, peaking at 6 h, then reducing little by little and coming to the level of the contrast; moreover the activity of LOX after BSM treatment was 7.81 U·g-1FW· min-1 which was restrained evidently, reducing to 75% of no BSM treatment at maximum value in 6 h. The ODC variation trend was similar to that of LOX; but the time of ODC peak was later than that of LOX, which separately was 41.12 CO2/g-1FW·min-1 and 36.50 CO2/g-1FW·min-1 at twelfth hour after topping. Then the activity of LOX descended laggardly, but in 48 h both of them were higher that the activity of the contrast.Key words: bionics signal molecule, tobacco alkaloids, topping, dynamic changesIV中国科学技术大学学位论文原创性声明本人声明所呈交的学位论文,是本人在导师指导下进行研究工作所取得的成 果。除已特别加以标注和致谢的地方外，论文中不包含任何他人已经发表或撰写 过的研究成果。与我一同工作的同志对本研究所做的贡献均已在论文中作了明确 的说