三九肠舒胶囊治疗肠易激综合征的实验分析-experimental analysis of sanjiu changshu capsule in treating irritable bowel syndrome.docx

model and atropine model mice. Then determine the gastric residual rate. In bowel driving experiments, we do the same but determine the bowel driving rate. The in vitro experiments include the normal, Ach spasm and barium chloride spasm models. We administrate the medicated serum of SC five days to the colon and rectum to the normal, Ach and barium chloride rats. Then determine the inhibition rate of the medicine to the tension of colorectal smooth muscle.3. Experiments of curing diarrhea and relieving pain. In the experiment of curing diarrhea, we set up the model by administrating the mice with castor oil or folium senna and observe the amount of loose stool within six hours. The experiment of relieving pain includes the hot plate and stretching model. We observe the pain leak point and its incubation period and the stretching times.The experimental data above are all analyzed by analysis of variance with SPSS 11.0.Results:1. SC has cured the hepatosplenic disorder disease-syndrome combined IBS rats through degrading the colorectal sensitivity, shortening the time when the glass ball out, cutting down the level of SP, MTL and stepping up the level of VIP in blood plasma and decreasing the level of Gas in blood serum. SC has treated the acetic acid or mustard IBS rats through decreasing the colorectal sensitivity, increasing the leak points of abdomen raising and back arching, degrading the frequency, peak potential, peak-bottom value and the area of the gastrointestinal electrical activity.SC has the two-way regulation effect. It can decrease the gastric residual rates and the bowel driving rates of the normal and neostigmine mice and increase the two indicators of the normal and atropine mice.Also, SC has the spasmolysis effect through inhibiting the tension of colorectal smooth muscle of the normal, Ach spasm and barium chloride spasm rats.SC cured diarrhea through decreasing the amount of loose stool of the castor oil or folium senna mice. SC relieved pain by