感毒清口崩片对免疫抑制小鼠免疫调节作用的实验分析-experimental analysis of the immunoregulatory effect of ganduqing orally disintegrating tablets on immunosuppressed mice.docxVIP

关键词：感毒清口崩片免疫抑制免疫增强作用ABSTRACTObjectIn recent years,With the period of the increasing in atmospheric pollution and aged tendency of population , respiratory diseases caused more and moreattention ,Chinese medicine can benefit the human body, improve human immunity, enhance the body fighting the infection of pathogenic microorganisms, and play an important role in the prevention and treatment of respiratory diseases.This experiment of animal experimental illustrates the new drug Ganduqing buccal tables can enhance immunity, expounds its popularization in clinical, the feasibility of the prevention and treatment of influenza virus infection .MethodsThe study uses randomized,controlled,double-blind experimental design methods.Purchase 60 ordinary level Kunming mice.Equal number of male and female. all mice were randomly divided into 6 groups,except the normal control group.Inorder to cause immune suppression model, injecting cyclophosphamide 80ml/kg, once a day for three consecutive days,GDQ in high dose,middle dose and low dose,yupingfengsan(a TCM decoction)Gavage once daily for seven consecutive days,Using flow cytometry to observe NKT cells , Th17 cells percentage changes in the blood of mice.By enzyme-linked immunosorbent assay(ELISA) to research cell factor β-defensin-2, IFN-γ,IL-2,IL-4 content changes in the thymus tissue homogenate.Results1comparing with cyclophosphamide model group,after gavage ganduqing(GDQ) to mice for 7 days,NKT cells were significantly increased in the blood of mice and showed a dose-effect relationship,The effect of the high-dose group is most obvious. percentage of Th17 cells in CD4 + T tended to increase in blood, but no statistically significant (P 0.05).2gavage ganduqing(GDQ) for 7 days, then take the thymus tissue make into thymus tissue pared with cyclophosphamide model group,The levelof βD-2、IFN-γ、IL-2 significantly increased,and IL-4 obviously reduced.(P0.05).ConclusionIn the state of immunity is suppressed,gavage ganduqing(GDQ) to t