干旱诱导黄瓜幼苗对低温胁迫的交叉适应性分析-analysis of cross adaptability of drought-induced cucumber seedlings to low temperature stress.docxVIP

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干旱诱导黄瓜幼苗对低温胁迫的交叉适应性分析-analysis of cross adaptability of drought-induced cucumber seedlings to low temperature stress.docx

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干旱诱导黄瓜幼苗对低温胁迫的交叉适应性分析-analysis of cross adaptability of drought-induced cucumber seedlings to low temperature stress

干旱诱导黄瓜幼苗对低温胁迫的交叉适应性研究chilling damage degree of cell membrane in cucumber seedlings.Chilling stress for 7 d let to injury in the cell membrane and chloroplast membrane the control leaves, vanish in grana lamella, derease in size and number of starch grains, accumulate in osmiophilic grains, dissolve in mitochondrial membrane. However, the PEG pretreatment showed complete membranes of mitochondria and chloroplasts approximately, distincted grana lamella, excepted for injury in a part of the plasma membranes, and alveolation in mitochondria. This reveals that PEG pretreatment plays an important role in stabilization of cell structure in cucumber leaves.PEG pretreatment showed significant higher ABA content, lower GA3 content and the ratio of GA3/ ABA in cucumber seedlings before and after chilling stress. Higher ABA content and lower GA3/ ABA ratio is one of the internal causes in increase of cold tolerance in PEG pretreated seedlings.The results of the experiment in soil cultivation showed that the electrolyte leakage and chilling injury index of drought pretreated seedlings were lower obviously than those of the control after chilling stress for three days. Recovery in optimal condition for three days, the drought pretreatment was close to normal, whereas the control seedlings showed significant higher electrolyte leakage than before chilling stress days.Key words: Cucumber; Cross adaptation to drought-low temperature; Osmoregulation ability; Photosynthetic capacity; Antioxidant capacity; Cell structure; Hormone4英文缩写符号及中英文对照表英文缩写ABA英文名称Abscisic acid中文名称脱落酸APXAscorbate peroxidase抗坏血酸过氧化物酶AsAAscerbic acid抗坏血酸CaCalcium钙CATCatalase过氧化氢酶℃Degree centigrade摄氏度Chl aChlorophyll a叶绿素 aChl bChlorophyll b叶绿素 bCiCKIntercellular CO2 concentrationControl胞间 CO2 浓度对照CO2DTCarbon dioxideDrought treatment二氧化碳干旱胁迫Fv/FmMaximal photochemical efficiency of PSIIPSII 最大光化学效率Fv′/Fm′Antenna efficiency of PSIIPSII 天线色素捕光效率GAGibberellin赤霉素GRGlutathione reductase谷胱甘肽还原酶GsStomatal conductance气孔导度hH