parp1抑制剂作为131i辅增敏剂在分化型甲状腺癌治疗中的应用-application of par p1 inhibitor as 131 i adjuvant sensitizer in the treatment of differentiated thyroid cancer.docxVIP

英文缩略词表缩写英文全称中文对照PARP-1poly ADP-ribose polymerase 1聚腺苷酸二磷酸核糖转移酶-1Bcl-2 B-cell lymphoma-2 B 淋巴细胞瘤-2 基因PTC Papillary thyroid carcinoma 乳头状甲状腺癌 FTC Follicular thyroid carcinoma 滤泡状甲状腺癌 ATC Anaplastic thyroid carcinoma 未分化甲状腺癌MTCMedullary thyroid carcinoma甲状腺髓样癌 DTCDifferentiated thyroid cancer分化型甲状腺癌 RAITRadioactive Iodine Treatment放射碘治疗TSH Thyroid stimulating hormone 血清促甲状腺激素 TPO Thyroid peroxidase 甲状腺过氧化物酶 NIS Sodium-iodide symporter 钠/碘同向转运体摘要目的:探讨 131I 照射以及联合 PARP-1 抑制剂对于分化型甲状腺癌细胞系 BCPAP增殖与凋亡的影响.方法 常规培养人分化型甲状腺癌 BCPAP,采用 CCK-8 法检测 BCPAP 的增殖和流 式细胞术检测细胞凋亡,并用 Western-blot 技术检测 Bcl-2,Bax 的表达.结果 131I 和 PJ-34 均能抑制 BCPAP 细胞的增殖,且与浓度呈相关性,而且二者联合 作用比单独应用 131I 有更好的抑制效果.流式细胞术检测结果显示 131I 和 PJ-34 的 联合作用比单独用 131I 有更高的凋亡率.Western-blot 显示随着 131I 中加入的 PJ-34 浓度的升高,Bax 表达量逐渐升高,而 Bcl-2 的表达量呈降低趋势.结论 131I 和 PJ-34 联合作用比单独应用 131I 对 BCPAP 有更好的抑制效果和更强 的促凋亡作用,这为 131I 和 PJ-34 联合治疗分化型甲状腺癌提供了初步的实验依 据.关键词 分化型甲状腺癌; 细胞增殖 ;细胞凋亡; I-131;PJ-34.Abstractbstract Objective To investigate the influence of I-131 combine with parp-1 inhibitor (PJ-34) on the proliferation and apoptosis of BCPAP cells.Methods The BCPAP cells were routinely cultured,CCK-8 was used to analyze cell proliferation, apoptosis was assessed by flow cytometry, the expression of Bcl-2and Bax was detected by using Western blot.Results The I-131 and PJ-34 both suppressed the BCPAP CELLS correlated with the concentration, and I-131 combine with PJ-34 could suppress the proliferation stronger than I-131 does. I-131 combine with PJ-34 also have high apoptosis rate than I-131 does by flow cytometry , the western-blot illustrate that the expression of Bax increased and the expression of Bcl-2 decreased with PJ-34 exaltation in BCPAP cells of I-131.Conclusion I-131 combine with PJ-34 have better suppression and stronger apoptosis of BCPAP cells than only I-131 does .our study provided a preliminary evidence by using I-131 combination with PJ-34 for thyroid cancer threatment.Key Word Thyroid cancer, Cell proliferation ,Cell apoptosis