osahs患者rage表达以及血清age srage和esrage水平与胰岛素抵抗和血压关系-relationship between rage expression, serum age sr age and es rage levels and insulin resistance and blood pressure in osahs patients.docxVIP

第三部分 OSAHS 患者血清 AGE、esRAGE 和 sRAGE 水平与血压的关系目的 晚期糖化终末产物（AGE）与其细胞表面受体（RAGE）的相互作用在心血 管疾病和糖尿病并发症发病机制中起着重要作用。RAGE 的两个可溶性类型：可 溶性 RAGE（sRAGE）和内源分泌型 RAGE（esRAGE）可能通过作为受体诱饵的作 用，可能阻断 RAGE 信号通路的激活。本研究旨在探讨阻塞性睡眠呼吸暂停低通 气综合征（OSAHS）患者血清 sRAGE 和 esRAGE 水平与血压的关系。方法 本研究纳入 139 例非糖尿病男性 OSAHS 患者和 53 例正常对照者。血清 AGE、 sRAGE 和 esRAGE 水平采用酶联免疫吸附法（ELISA）测定，早晨连续 3 次坐位血 压（SBP 和 DBP）测定。结果 单因素相关性分析显示，血清 esRAGE 水平与 SBP 和 DBP 显著相关，但血 清 sRAGE 水平与 SBP 和 DBP 均无显著相关性。多元回归分析显示，OSAHS 患者 SBP 与腰围、HbA1c 和最低 SaO2 呈显著性独立正相关，与血清 esRAGE 呈显著性 独立负相关；DBP 与 LDL-C、AHI、血清 AGE 和 BMI 显著性独立正相关，但与血 清 esRAGE 无显著性相关。结论 本研究结果显示，非糖尿病男性 OSAHS 患者血清 esRAGE 与血压显著负相 关，尤其与 SBP。从而提示 esRAGE 可能在 OSAHS 患者高血压中起到一定的保护 作用，也可能是评估 OSAHS 患者处于心血管疾病高风险的新的生物标志物。关键词：晚期糖化终末产物受体（RAGE）；内源分泌型 RAGE；阻塞性睡眠呼吸 暂停低通气综合征；高血压ABSTRACTPart 1. Expression of RAGE, and serum AGE ， esRAGE and sRAGE levels in nondiabetic patients with OSAHSObjective Obstructive sleep apnea hypopnea syndrom (OSAHS) may cause oxidative stress and inflammation reaction, and induced production of advanced glycation end products (AGE). Interaction of advanced glycation end products (AGE) and their specific cell-surface receptor (RAGE) play an important role in the pathogenesis of cardiovascular disease and diabetic complications. Two isoforms of C-truncated RAGE, esRAGE and sRAGE, may prevent activation of RAGE signaling by actingas decoys. This study investigated the expression of RAGE, and serum AGE ，esRAGE and sRAGE levels in nondiabetic patients with OSAHS.Methods Male nondiabetic patients (n=139) with OSAHS and controls without OSAHS (n=53) were enrolled, of which 13 nondiabetic patients with OSAHS and 13 controls without OSAHS were detected for the expression of RAGE in peripheral blood mononuclear cells (PBMC). The expression of RAGE mRNA and protein were detected by quantitative PCR and western blotting respectively. Serum AGE, esRAGE and sRAGE levels were examined using enzyme-linked immunosorbent assay.Results Serum AGE level and the expression of RAGE mRNA and protein in PBMC