一种嵌合型调控元件在肿瘤靶向基因治疗中应用-application of chimeric regulatory element in tumor target gene therapy.docxVIP

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一种嵌合型调控元件在肿瘤靶向基因治疗中应用-application of chimeric regulatory element in tumor target gene therapy.docx

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一种嵌合型调控元件在肿瘤靶向基因治疗中应用-application of chimeric regulatory element in tumor target gene therapy

目录摘要 ·······························································································Ⅲ ABSTRACT ·······················································································Ⅳ 图录 ·································································································Ⅴ 表录 ·································································································Ⅵ 第一章绪论 ······················································································· 7 1.1 靶向治疗中翻译起始的调控 ··························································· 71.2 靶向治疗中转录水平的调控 ··························································· 81.3 靶向治疗中转录后水的调控 ··························································· 9 第二章实验材料与方法 ·······································································12 2.1 实验材料 ··················································································122.1.1 细胞系 ················································································122.1.2 主要试剂 ·············································································122.1.3 主要试验仪器 ·······································································132.2 常用实验操作 ············································································142.2.1 细胞培养 ·············································································142.2.2 流式细胞分析 ·······································································152.2.3 细胞转染和 MTT 实验 162.2.4 荧光素酶检测 ·······································································172.2.5 RNA 提取、RNA 测定 172.2.1 DNA 凝胶电泳 182.2.3 RNA 反转录和 qPCR 192.2.7 质粒构建 ·············································································212.2.1 western 蛋白检测 24第三章结果 ······················································································25 3.1 构建嵌合型表达调控元件 ··························································253.2 前列腺癌细胞系中内源性基因的表达情况 ·····································283.2.1 survivin 在前列腺癌细胞系中