吲哚胺23-双加氧酶基因转染对肝癌细胞的凋亡及相关机制的实验研究-experimental study on apoptosis and related mechanisms of indoleamine 23 - dioxygenase gene transfection in hepatocellular carcinoma cells.docxVIP

(35.00±2.20)%,(74.51±1.44)%,(75.30±1.67)%(P0.05);andwith1-MTgroups,thatwererespectively(71.33±2.10)%,(81.27±2.42)%,(80.91±1.95)%;therewasasignificantincreaseinthecytotoxicityofT-lymphocyteagainstHepG2cellincoculturedconditionwith1-MTgroups.Afterfivedaysofcombinereaction,thepercentageofTregcellinIDO-HepG2groupwassignificantlyhigherthanwhichinpcDNA3.1-HepG2andHepG2groups.Theywererespectively（10.53±1.05）%，（4.03±0.17）%（,3.34±0.18）%(P0.05);inadding1-MTgroups,thatwererespectively（6.37±0.15）%，（1.43±0.10）%,（1.57±0.13）%,itwasconsideredstatisticallysignificantcomparedwiththecontrolgroupwithout1-MT.Inthemousemodelofhumanlivercancercells,thepercentageofTregcellinperipheralbloodofIDO-HepG2groupwassignificantlyhigherthanwhichinpcDNA3.1-HepG2andHepG2groups;theywererespectively（15.33±1.18）%，（8.96±0.53）%，（8.14±0.57）%;itwasconsideredstatisticallysignificantbetweentheover-expresstionofIDOgroupandothergroups(P0.05).Conclusions1、ThoughincreasingthepercentageofTregcellsinT-lymphocytes,IDOcansuppresstheapoptosisrateofhepatocellularcarcinomacellsandthecytotoxicityofT-lymphocyte.1-MTcanreversetheroleofIDO.2、Invivotest,itcanbeconfirmedthatover-expressionofIDOcanincreasetheproportionofTregcellsinperipheralblood.KeyWordsindoleamine2,3-dioxygenase,hepatocellularcarcinomacells,T-lymphocyte,Tregcells,apoptosis主要缩略词表HCCIDOD-1-MTCTLA-4hepatocellularcarcinomaindoleamine2,3-dioxygenaseD-1-methyl-tryptophancytotoxicTlymphocyte-associatedantigen-4肝细胞癌吲哚胺2,3-双加氧酶D-1-甲基色氨酸细胞毒T淋巴细胞相关抗原4NKnaturalkiller自然杀伤DMSOdimethylsulfoxide二甲基亚砜TNF-αtumornecrosisfactor-α肿瘤坏死因子-αMHCmajorhistocompatibilitycomplex主要组织相容性复合体CTLcytotoxicTlymphocyte细胞毒（性）T淋巴细胞LLClewislungcarcinomaLewis肺癌APCantigenpresentingcell抗原提呈细胞TregCD4+CD25+regulatoryTcells调节性T细胞PDCsplasmacytoiddendriticcells类浆细胞MAGEmelanomaantigen黑素瘤抗原IFN-γinterferon-γγ-干扰素学位论文独创性声明本人卜晓倩声明，所呈交的学位论文系在导师指导下本文独立完成的研究成果。文中任何引用他人的成果，均已做出明确标注或得到许可。论文内容未包含法律意义上已属于他人的任何形式的研究成果，也不包含本人已用于其他学位申请的论文或成果。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示谢意。本文如违反上述声明，愿意承担以下责任和后果：1、交回学校授予的学位证书；2、学校可