吲哚胺23-双加氧酶对效应cd8t细胞溶胞作用的免疫抑制作用-immunosuppressive effect of indoleamine 23 - dioxygenase on the lysis of cd8t cells.docxVIP

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吲哚胺23-双加氧酶对效应cd8t细胞溶胞作用的免疫抑制作用-immunosuppressive effect of indoleamine 23 - dioxygenase on the lysis of cd8t cells.docx

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吲哚胺23-双加氧酶对效应cd8t细胞溶胞作用的免疫抑制作用-immunosuppressive effect of indoleamine 23 - dioxygenase on the lysis of cd8t cells

山西医科大学硕士学位论文山西医科大学硕士学位论文 PAGE PAGE IV respectively 1.38±0.017、1.21±0.021、1.32±0.027), it was considered no statistically significant comparing the former with the latter two (P0.05); adding 1-D-MT (concentration of 2.5mmol/l ) to the I group(I+1-D-MT group) （1.39±0.016）did not reverse the granzyme B mRNA expression of effector CD8+ T lymphocyte, it was considered no statistically significant comparing the I group with the I+1-D-MT group(P0.05). The granzyme B protein expression of effector CD8+ T lymphocyte in each group was detected by Western blot: The granzyme B protein expression of effector CD8+ T lymphocyte in the I group(0.52±0.017) was significant lower than in the P group and the 7721 group(1.02±0.023、1.15±0.055 respectively), it was considered statistically significant comparing the former with the latter two (P0.05); after 1-D-MT (concentration of 2.5mmol/l ) was added to the I group(I+1-D-MT group), the granzyme B protein expression of effector CD8+ T lymphocyte(1.01±0.025) was higher than the I group(0.52±0.017), it was considered statistically significant comparing the I group with the I+1-D-MT group(P0.05). The cytolytic activity of effector CD8+ T lymphocyte against SMMC-7721 cell was detected by LDH assay kit: A significant decrease of cytolytic activity was observed in the I group (15.32±4.06%) over the P group and the 7721 group(60.37±1.53%、60.88±1.49%), it was considered statistically significant comparing the former with the latter two (P0.05); after 1-D-MT (concentration of 2.5mmol/l ) was added to the I group(I+1-D-MT group), the cytolytic activity of effector CD8+ T lymphocyte (60.34±1.23%)was higher than the I group(15.32±4.06%), it was considered statistically significant comparing the I group with the I+1-D-MT group(P0.05). Conclusions The IDO mRNA and IDO protein was expressed by the SMMC-7721 cell transiently transfected with recombinant plasmid pcDNA3.1-IDO, and the 1-D-MT inhibit the expression of the IDO