玉米抗旱性及产量相关性状的qtl研究-qtl study on drought resistance and yield-related traits in maize.docxVIP

玉米抗旱性及产量相关性状的QTL分析mapsinchromosomebinlocus,thelinkagemapestablishedinthisstudywasconsistentwiththem.TheSSRlinkagecanbeusedforQTLmapping.Bycompositeintervalmapping(LOD3.0),68QTLsweredetectedfor6traitsundertwowaterregimes.Underwaterstressenvironment,37QTLsweredetected;andundernorm-alirrigationenvironment31QTLsweredetected.Eachtraithasbeenidentified2-4QTLswhichwereresponsibleforinterpreting8.09-13.97%ofthephenotypicvarianceindividually.QTLsofplantkernelnumberperrowandgrainyieldperplantarerelativelyconsistenceacrosstwoenvironments.ThisresultalsoshowthatsomeQTLstrendtoclusterthesamechromosomeregion.Thekeychromosomeregionsfordroughtresistancemostlylieonthefollowingchromosomes:chromosome2,umc1580aandbnlg121;chromosome4,umc2280andumc1652;chromosom-e9,umc1494andumc1657.ThemostimportantQTLeffectsweredetectedonchromosome2,4and9forgrainyieldandyieldcomponentsunderNIandDStreatmentsattwosites.TheQTLscontaingenescontrollingseveraltraitsanditisimportanttoaddressherethatinthiscasetheseQTLsareextremelystable.ThepresenceoftheseQTLsmaycontributetofine-mapping,identificationofcandidategenesandMASstrategiesformaizeimprovementpurposesunderphosphorusdeficiencycombiningtraditionalbreedingapproaches.Keywords:Maize;Droughtresistance;Yield-relatedtrait;QTL;SSR符号说明ANOVA:Analysisofvariance，方差分析ASI:anthesis-silkinginterval，雌雄穗开花间隔CIM:compositeintervalmapping，复合区间作图cM:centimorgan，分摩尔根（基因交换单位）DS:droughtstress，干旱ED:eardiameter，穗粗EL:earlength，穗长GY:grainyieldperplant，单株产量KN:kernelnumberperrow，行粒数LOD:likelihoodratiooroddsratio，似然比MAS:markerassistedselection，分子标记辅助选择NI:normalirrigation，正常灌溉PH:plantheight，株高QTL:QuantitativeTraitLoci，数量性状位点SSR:simplesequencerepeat，简单重复序列关于学位论文原创性和使用授权的声明本人所呈交的学位论文，是在导师指导下，独立进行科学研究所取得的成果。对在论文研究期间给予指导、帮助和做出重要贡献的个人或集体，均在文中明确说明。本声明的法律责任由本人承担。本人完全了解山东农业大学有关保留和使用学位论文的规定，同意学校保留和按要求向国家有关部门或机构送交论文纸质本和电子版，允许论文被查阅和借阅。本人授权山东农业大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或其他复制手段保存论文和汇编本学位论文，同时授权中国科学技术信息研究所将本学位论文收录到《中国学位论文全文数据库》，并向社会公众提供信息服务。保密论文在解密后应遵守此规定。论文作者签名：导师签名：日期：1前言玉米（ZeamaysL.）属禾本科