nm23和p53在宫颈癌组织中表达及意义.docVIP

nm23和p53在宫颈癌组织中表达及意义 　　[摘要] 目的：探讨肿瘤抑制基因nm23与p53的蛋白产物在宫颈癌组织中的异常表达及其与肿瘤的临床分期、病理分级和淋巴结转移等生物学特征的关系。方法：应用免疫组化方法检测60例宫颈癌手术切除标本和15例宫颈正常组织中nm23和p53的表达情况。结果：nm23和p53蛋白表达量在宫颈癌的分化程度、临床分期及局部淋巴结转移方面存在显著性差异，两者呈负相关。结论：nm23和p53蛋白在宫颈癌的演进中，尤其在宫颈癌的浸润和转移方面，很可能起着相互促进的作用。因此，联合检测nm23和p53蛋白的表达可提高宫颈癌的转移及预测临床诊治的准确性。 　　[关键词] 宫颈癌；nm23蛋白；p53蛋白 　　[中图分类号] R737.33[文献标识码]A [文章编号]1673-7210（2009）06（c）-007-02 　　 　　Expression and significance of nm23 and p53 in cervical cancer 　　LI Rongli1, LIU Entong2 　　(1.Department of Obstetrics and Gynecology, the Fourth Peoples Hospital of Lanzhou, Lanzhou 730050, China; 2.Department of Pathology, the Fourth Peoples Hospital of Lanzhou, Lanzhou 730050, China) 　　[Abstract] Objective: To study the abnormal expressions of tumor suppressor nm23 and p53 protein in cervical cancer, and their relationships with tumor biological characteristics including clinical stages, pathological grades and lymph node metastasis and so on. Methods: Immunohistochemical technique was used to examine the expression of nm23 and p53 in 60 cases of cervical squamous cell carcinoma and 15 cases of normal cervical tissues. Results: The expression of nm23 showed significant difference in tumor grades, clinical stages and local lymph node metastasis compared with p53, and they were negatively correlated. Conclusion: nm23 and p53 protein may play an important role in the development of cervical carcinoma, they promote mutually especially in tumor invasion and metastasis. The combined detection of the expression of nm23 and p53 protein can be used to estimate metastatic potentiality and predict the accuracy of clinical treatment. 　　[Key words] Cervical cancer; nm23 portein; p53 portein 　　 　　宫颈癌是全球妇女中仅次于乳腺癌的第二个最常见的恶性肿瘤。癌基因的激活及抑癌基因的失活是细胞癌变的分子基础。nm23是第一个被发现的转移抑制基因，其异常表达与多种肿瘤的转移及预后相关。众所周知，p53基因是与肿瘤关系最为密切的肿瘤抑制基因，60％以上的肿瘤存在p53基因异常。本研究采用免疫组化技术检测了nm23及p53蛋白在宫颈癌组织及正常组织中的表达情况，以探讨其在宫颈癌的发生、发展中的作用及相互关系。 　　1材料与方法 　　1.1标本 　　收取兰州大学第一医院和兰州市第四人民医院2003～2005年手术切除宫颈癌的石蜡包埋标本共60例。患者年龄26～74岁，平均59.7岁。其中，高分