SD大鼠实验性变态反应性脑脊髓炎模型建立.docVIP

SD大鼠实验性变态反应性脑脊髓炎模型建立 　　【中图分类号】 R331 514 【文献标识码】 A 【文章编号】1185-1672(2006)-08-0676-06 　　摘要 目的 探索SD大鼠实验性变态反应性脑脊髓炎(EAE)模型制作的试验方法与技术。方法 采用完全福氏佐剂－脑脊髓匀浆，并辅以注射百日咳疫苗诱导SD大鼠EAE模型，比较不同的免疫原（同种或异种），注射方法（一次或多次注射）以及性别差异对模型制作的影响。观察和比较动物的神经症状和病理改变。结果 使用同种脑脊髓匀浆，多次注射雌性SD大鼠诱导的模型，其发病持续时间分别为16～20天，10～14天；发病率为85％；复发率为46 67%，而致死率仅有5 88％；明显优于其他组别。病理学证实发病大鼠具有典型的脱髓鞘改变和胶质结节形成。结论 使用同种脑脊髓匀浆，多次注射雌性SD大鼠诱导的动物模型其临床表现、病理特征、病程特点与人类的多发性硬化（MS）具有很大的相似性，适用于MS的??究。 　　关键词 实验性变态反应性脑脊髓炎；SD大鼠；动物模型 　　 　　The establishment of experimental allergic encephalomyelitis model in SD rat 　　Zheng JianZheng,Gao Cong,Xie FuHua,et,al 　　The Second Afficiated Hospital of Guangzhou Medical College,Guangdong,510260,China 　　Abstract Objective To explore the experimental method and technique of making experimental allergic encephalomyelitis model in SD rat Methods The animal model was established in SD rat by injecting spinal cord homogenate in Freuds complete adjuvant and bordetella pertussis vaccine(BPV)We had used different methods such as homologous or heterologous homogenate, one or multiple injections, and different rats to establish animal modelIts clinical symptoms and histological alteration was observed and comparedResultsThe animal model by injecting homologous homogenate twice in female SD rat is better than other, whose duration of paralysis is 16～20 days，and incidence 85％, incidence of relapse 46 67%，and fatality rate 5 88％We could find many demyelinated lesions in the brain and spinal cord in animal like human with multiple sclerosis(MS)Conclusion The animal model of EAE induced by injecting homologous homogenate twice in female SD rat bears a resemblance to the features of MS, and might be a better model for the study of multiple sclerosis 　　Key Words Experimental allergic encephalomyelitis; SD rat; Animal model 　　 　　实验性变态反应性脑脊髓炎（experimental allergic encephalomyelitis, EAE）是实验敏感动物发生的,由同种型，同种异型，异种异型致脑炎抗原诱导产生的，由细胞免疫反应介导的，以中枢神经系统白质脱髓鞘性为特征的迟发型超敏反应性自身免疫疾病。EAE具有与人类多发性硬化（Multiple Sclerosis，MS）极为相似的临床，生化，免疫及病理特