β-纤维蛋白原-455GA基因多态性与缺血性脑血管疾病相关性研究.docVIP

β-纤维蛋白原-455GA基因多态性与缺血性脑血管疾病相关性研究 　　［摘 要］ 目的 研究β_纤维蛋白原_455G/A(β_Fg_455G/A)基因多 态性对血浆纤维蛋白原(fibrinogen, Fg)基因表达的影响及其与缺血性脑血管疾病发病的关 系。方法 应用聚合酶链反应(PCR)及限制性片段长度多态性分析(RFLP)等 方法，检测DNA样品的β_Fg_455G/A多态性，采用血浆自动检测系统测定Fg水平。结 果 病例组A等位基因频率明显高于对照组(P＜0.05)。病例组血浆Fg水平明 显高于对照组(P＜0.01)，病例组和对照组中，携带A基因者血浆Fg水平明显增高(P ＜0.05)。结论 β_Fg_455G/A等位基因与血浆纤维蛋白原水平相关，A 基因携带者缺血性脑血管疾病发病率增高。提示β_Fg_455G/A可作为缺血性脑血管疾病的遗 传易感标志之一用于易感人群的检测。 　　［关键词］ 纤维蛋白原；基因多态性；缺血性脑血管病 　　中图分类号：R743；R197 文献 标识码：A 文章编号：1009_816X(2007)04_0232_ 03 　　Study on the relationship between β_Fibrinogen_455G/A Polymorphism a nd Ischemic Cerebral Vascular Disease. CHENG Jun-ying, LU Li-ping, DU Xiao-peng, et al. Department of neurology, Lishui Peoples Hospital, Zhejiang 3 23000, China 　　［Abstract］ Objective To study the relationship between β_fibr inogen_455G/A polymorphism, plasma fibrinogen level and ischemic cerebral vacsul ar disease. Methods Study included 82 patients with ischemic cer ebral vascular disase (P group) and 62 healthy people (C group). For all subject s, the polymorphism of β_fibrinogen_455G/A was analyzed by polymerase chain rea ction and restriction fragment length polymorphism (PCR-RELP) technique. Plasma fibrinogen was measured by a computer assistant procedure. Results The frequency of -455A allele in P group was higher than in C group (P＜0 .05). The plasma fibrinogen level was significantly higher in P group than in C group (P＜0.01). Higher plasma fibrinogen level was shown in all subjects with A-455 alleles (P＜0.05). Conclusions The β_fibrinogen _455G/A allele is associated with elevated fibrinogen level and those with A all ele are prone to suffer ischemic cerebral vascular disease. 　　［Key words］ Fibrinogen; Gene polymorphism; Ischemic cerebral v ascular disease 　　 　　随着我国老龄化社会的到来，缺血性脑血管疾病的发病率呈逐年上升趋 势。血浆纤维蛋白原(fibrinogen, Fg)水???升高被认为是缺血性脑血管疾病的独立危险因素 之一［1］。目前研究多认为，Fg基因的多态性，尤其是其启动子区域的β_Fg_455G/ A基因多态性与血浆Fg水平密切相关，但与缺血性脑血管疾病的相关性有相反的报道［ 2，3］。本研究旨在观察β_Fg_455G/A基因多态性对血浆Fg及缺血性脑血管疾病发病的影 响，探讨Fg增高及缺血性脑血管疾病发病的可能机制，为缺血性脑血管