人生长激素对人胃结肠癌细胞株细胞周期影响.docVIP

人生长激素对人胃结肠癌细胞株细胞周期影响 　　【摘要】 目的 了解重组人生长激素(rhGH)在体外对人胃、结肠癌细胞有无促进增殖作用。方法 实验分对照组、rhGH组、奥沙利铂(L-OHP)组和L-OHP+rhGH组,利用体外细胞培养及流式细胞仪等方法,测定不同浓度的rhGH对人胃癌细胞株MKN45、人结肠癌细胞株Colo320细胞周期和增殖指数(PI)的影响。结果 rhGH在体外不促进MKN45和Colo320细胞分裂增殖。rhGH组与对照组、L-OHP+rhGH组与L-OHP组比较均无统计学意义(P0.05),L-OHP+rhGH组与对照组比较或L-OHP+rhGH组与对应的rhGH组比较,阻滞于G0~G1期的细胞数增加,S期和G2~M期细胞明显减少,PI明显降低(P 　　【关键词】重组人生长激素;胃肿瘤;结肠肿瘤;细胞周期 　　 　　In vitro effects of recombinant human growth hormone on cell cycle of human gastric and colonic cancer cell line 　　 　　HUANG Shi-hua,CHENG Jia-yong,ZENG De-miao.Department of General Surgery, 　　The Fristh People’s Hospital of Zhaotou,Zhaotong 670000 YunNan,China 　　 　　【【Abstract】 Objective To study effects of recombinant human growth hormone(rhGH)on proliferation of human gastric and colonic cancer cell in vitro.Methods After experiment were divided into control group,rhGH group,oxaliplatin(L-OHP)group and L-OHP+rhGH group,Cell cycle and Proliferation Index(PI)of human gastric and colonic cancer cell line,MKN45 and Colo320,were determined in different concentration of rhGH.Results In vitro the distincteffects of rhGH on proliferation of cell line,MKN45 and Colo320 were not found.There were not statistical significance compared rhGH group with control group or compared L-OHP+rhGH one with L-OHP one(P0.05).The cells arrested in G0~G1 phase obviously increased,meanwhile,the cells in S phase and G2~M phase and PI distinctly decreased compared L-OHP+rhGH group with control group or between L-OHP+rhGH group and rhGH group in comparison(P0.05)。S期:L-OHP组和L-OHP+rhGH组比对照组和rhGH组降低(P0.05),但有升高趋势。G2~M期:L-OHP组和L-OHP+rhGH组比对照组和rhGH组降低(P0.05);同时,L-OHP+rhGH组与对应的rhGH组及对照组比较,G2~M期细胞也减少(P 　　 　　表2 　　 　　药物作用后MKN45和Colo320细胞株各组的增殖周期百分数( x±s) 　　 　　组别 　　MKN45(%)Colo320(%) 　　 　　G0~G1SG2~MG0~G1SG2~M 　　 　　对照组51.5±1.736.2±1.512.3±3.325.6±3.240.1±1.634.2±4.4 　　L-OHP组81.8±1.5?*8.9±0.7?*9.3±1.257.4±5.4?*25.3±2.8?*17.3±3.4?* 　　rhGH1组54.6±3.433.5±6.511.9±3.326.4±2.539.9±1.533.6±2.7 　　rhGH2组53.8±4.13