卡培他滨对食管细胞系shee及sheec作用分析-effect of capecitabine on shee and sheec cell lines.docxVIP

cells using with 10% blood serum contained drugs were 11.23% and 23.63% at 48h, it was significantly different (P0.05); The growth inhibiting ratios of SHEE and SHEEC cells using with 10.375ng/ml Fluorouracil, which concentration was same as Fluorouracil in the group of 10% blood serum contained drugs, were 10.57% and 9.98% at 48h, it was not significantly different (P0.05); the growth inhibiting ratios of SHEE and SHEEC cells using with 227.8ng/ml Doxifluridine, which concentration was same as Doxifluridine in the group of 10% blood serum contained drugs, were 1.69% and 16.76% at 48h, it was significantly different (P0.05); the growth inhibiting ratios of SHEE and SHEEC cells using with 10.375ng/ml Fluorouracil plus 227.8ng/ml Doxifluridine, which concentration was same as Fluorouracil plus Doxifluridine in the group of 10% blood serum contained drugs, were 1.69% and 16.76% at 48h, it was significantly different (P0.05).Conclusion: Cancerization of normal esophageal epithelium cell follows with increase of positive expression of TP. Capatibine has weak inhibition effect to SHEE cell line, selective lethal effect to SHEEC cell line. Expression of TP have no obvious correlation with chemo effect of 5-Fu.KEY WORDS: human esophageal cell line, capecitabine, thymidine phosphorylase, Fluorouracil, DoxifluridineDissertation Type: Basic type for applicationIV缩略语词汇表SHEEShantou human embryonic esophageal epithelial cell line人胚食管上皮细胞汕头株SHEECShantou human embryonic esophageal carcinoma cell line人胚食管上皮癌细胞汕头株TP5-Futhymidine phosphorylase Fluorouracil胸苷磷酸化酶 氟尿嘧啶5-DFURDoxifluridine脱氧氟尿苷MTT M199Methylthiazoletrazolium Culture medium 199四甲基偶氮唑盐M199 培养基PBSPhosphate buffered saline磷酸盐缓冲液EDTAEthylene diaminetetraacetic acid乙二胺四乙酸DMSODimethyl sulfoxide二甲基亚砜ODOptical density光密度hhour小时33第1章 综述胸苷磷酸化酶与恶性肿瘤关系的研究进展恶性肿瘤已成为对人类健康危害最大的疾病之一，其发生发展是多种因素共 同作用的结果，如环境的恶化、遗传基因位点的突变以及机体免疫功能的下降 等。恶性肿瘤新生血管的形成、生长在恶性肿瘤生长、浸润和转移过程中所起的 作用已越来越受到重视。功能性血管为恶性肿瘤的生长提供氧和营养成分、保证 其在局部生长的同时，也为肿瘤细胞的扩散提供途径，有研究资料显示，除非新 生血管形