Hollow chitosan-alginate multilayer microcapsules as drug 中空的壳聚糖-海藻酸钠多层微胶囊药物.pptVIP

LOGO Qinghe Zhao, PhD,a,b Baosan Han, PhD,c Zhaohai Wang, MS,c Changyou Gao, PhD,a,b,* Chenghong Peng, PhD,c Jiacong Shen, BAa Hollow chitosan-alginate multilayer microcapsules as drug delivery vehicle:doxorubicin loading and in vitro and in vivo studies aDepartment of Polymer Science and Engineering, Zhejiang University, Hangzhou, China 課程：功能性高分子材料 老師：謝慶東 學生：廖家汶 前言 肝細胞癌是全世界最常見的癌症之一，也是十分惡性的癌，如果未經治療，病人往往在半年之內可能會死亡。 在全球癌症死因中排名第五的肝癌，原因在於多數病人發現太晚，只有15%的病人可接受手術切除，而末期肝癌患者大多數都是慢性B型肝炎或C型肝炎演變到肝硬化合併肝癌，若以傳統化學治療藥物治療時往往無法發揮良好功效，因此找尋一個具有緩慢釋放的載體釋放藥物進入人體中，成為本研究目的之一。 * 前言 藥物的控制釋放技術對於當前來說是一項非常重要的研究，影響的範圍涵蓋了組織工程與身體修復。 早期至今對於疾病與癌症的控制通常都是以定期的口服等多次的投藥方式，不但藥效不持久，浪費藥物成本，而且容易因為藥物濃度過高造成傷害生物體之其餘組織或產生不良的副作用。 本研究利用多層包覆的方式，包覆DOX抗癌藥物進行活體內外的多項模擬測試，希望可以克服傳統給藥系統，使藥物可以緩慢釋放於生物體中。 * 實驗材料 Chitosan(115 cps) CMC sodium salts(30-80 cps) DOX Sodium alginate(250 cps) Rhodamine B isothiocyanate CaCO3(3-5 μm) * 實驗方法 CaCO3 CMC 0.5 mg/mL Chitosan 0.5 mg/mL Alginate 2 % glutaraldehyde at room temperature for 12 hours 0.2 M EDTA * 結果與討論-SEM Fig 1. Scanning electron microscopy images of (A) CaCO3(CMC) microparticles and (B) cross-sectional view of a particle. 3-5 3-5 μm 8.8 m2/g 49.0 m2/g * DOX loading Fig 2. Doxorubicin (DOX) concentrations in the microcapsule interiors and in bulk as a function of DOX feeding concentration. The data embedded in the figure represent the concentration ratios between the capsule interiors and the bulk. The inset shows the chemical structure of DOX. * CLSM Fig 3. CLSM images of (A) CaCO3(CMC)/(chitosan-alginate) /Rd-chitosan- alginate, and (B) CaCO3(CMC)/(chitosan-alginate) microcapsules loaded with DOX. The microcapsules were cross-linked by 2% glutaraldehyde for 12 hours. * TEM Fig 4. TEM images of CaCO3(CMC)/(chitosan-alginate) microcapsules before (A) and after DOX loading (B). The microcapsules were crosslinked by 2% glutaraldehyde for 12 hours. * In vitro release Fig 5. A, DOX cumulative released amount from the microcapsules versus the incubation time. B, The