过氧化氢和氨基酸混合液对谷胱甘肽发酵影响研究.docVIP

过氧化氢和氨基酸混合液对谷胱甘肽发酵影响研究 　　摘要：目的 考察过氧化氢和氨基酸混合液前体对突变株YF（ZnCl2r，Ethr）生产谷胱甘肽（GSH）的影响。方法摇瓶培养，在不同添加时间分别添加不同浓度的过氧化氢和氨基酸混合液前体，测得GSH浓度，细胞干重。结果发酵9 h时添加12 mmol/L氨基酸混合液，GSH浓度提高了99.6 %。而过氧化氢对GSH发酵的影响不大。发酵12 h添加0.3 mmol/LH2O2和18 mmol/L氨基酸混合液，GSH浓度提高了114 %。结论氨基酸混合液能显著促进胞内谷胱甘肽的累积，氨基酸混合液和过氧化氢协同作用可有效的促进胞内谷胱甘肽的累积。 　　关键词：过氧化氢；混合氨基酸；谷胱甘肽；发酵 　　中图分类号：TQ920.1文献标识码：A文章编号：1672-979X(2008)07-0019-04 　　Study on Effects of Mixed Amino Acids and Hydrogen Peroxide on Production of Glutathione by Saccharomyces cerevisiae 　　CHEN Shan, HE Xiao-xian, PAN Ya-lei 　　(College of Life Science and Engineering, Shaanxi University of Science and Technology, Xi’an, 710021, China) 　　Abstract：Objective To investigate the effects of mixed amino acids and hydrogen peroxide on the production of glutathione（GSH）by mutant strain YF（ZnCl2r, Ethr）. Methods At the shake flask level, the concentration of GSH and the weight of dry cell were measured with different adding amount of mixed amino acids and hydrogen peroxide at different time points. Results After fermentation for 9 hrs, mixed amino acids of 12mmol/L was added into the medium with the concentration of GSH to be increased by 99.6%. However, hydrogen peroxide could not obviously enhance the intracellular GSH level. After fermentation for 12 hrs, hydrogen peroxide of 0.3mmol/L and mixed amino acids of 18mmol/L were both added into the medium with the concentration of GSH to be increased by 114%. Conclusion Mixed amino acids can enhance the intracellular GSH accumulation in shaking flask culture and the synergy of mixed amino acids and hydrogen peroxide can also increase the intracellular GSH accumulation. 　　Key words：hydrogen peroxide; mixed amino acids; glutathione; fermentation 　　 　　谷胱甘肽（glutathione，GSH），即γ-L-谷氨酰-L-半胱氨酰甘氨酸，是由L-谷氨酸、L-半胱氨酸和甘氨酸经肽键缩合而成，具有γ-谷氨酰基和巯基生物活性的一种三肽化合物。本课题用H2O2刺激突变株YF（ZnCl2r，Ethr），研究它对GSH合成的影响。 　　微生物细胞是一个经济的体系，不会在胞内过量积累某一代谢产物。细胞只有处于不正常的生理状态下，出于保护自身的本能和抵御外界伤害时，才会对其代谢产物做出调整。人为提供适当的环境刺激，极有可能促进细胞合成目标代谢产物的能力。Riccillo[1]指出在极端环境下GSH对Rhiz