益骨胶囊含药血清的对共育体系中破骨细胞活性和凋亡的影响.doc

益骨胶囊含药血清对共育体系中破骨细胞活性和凋亡的影响 生旦堡生理盘查neseJournalofPathophysio1ogY2006,22(5):1001—1004 [文章编号]1000—4718(2006}05—1001—04 益骨胶囊含药血清对共育体系中破骨 细胞活性和凋亡的影响 张荣华,徐立群,傅淑平,杨丽 (暨南大学药学院中药学教研室,广东广州510632) * [摘要]目的:观察益骨胶囊含药血清在成骨一破骨细胞共育体系中对sD大鼠破骨细胞(0c)活性和凋亡 的影响.方法:(1)取1d龄sD大鼠颅骨分离培养成骨细胞(OB),取5d龄SD大鼠四肢股骨,胫骨分离培养OC,建立 细胞上清相通但细胞间不相互混杂的平面式成骨一破骨细胞共育体系,实验分为含药血清组和对照组;(2)将10月 龄sD雌性大鼠分为益骨胶囊灌胃组和生理盐水对照组,制备含药血清和对照血清;(3)重氮盐法检测抗酒石酸酸性 磷酸酶(TRAP)和光镜观察骨陷窝数;(4)光镜和荧光显微镜下观察共育体系中0c凋亡情况.结果:含药血清组在 48h,72h,96h对成骨一破骨细胞共育体系中OC分泌TRACP的活性均明显降低于对照组,OC的存活数明显低于对 照组,OC的捌亡率明显高于对照组且呈明显的时效关系;所形成骨吸收陷窝的数目明显低于对照组(Plt;0.O1).结 论:益骨胶囊含药血清在共育体系中能够抑制OC活性,诱导破骨细胞的凋亡. [关键词]益骨胶囊;破骨细胞;细胞凋亡 [中图分类号]R363[文献标识码]A EffectofBBC-—containingserunlontheactivityandapoptosisofosteoclasts inosteoblastsandosteoclastsCO——culturesystem ZHANGRoflg—hua,XULi—qun,FUShu—ping,YANGLi (DepartmentofTraditionalChineseMed/c/ne,P/mrmacyCo//egeJinanUmversity,Guangzhou510632,China) [ABSTRACT]AIM;TostudytheeffectofBone—benefitingcapsule(BBC)一containingsen.1/nonactivityandapoptosis ofosteoclasts(0C)inosteoblasts(0B)andosteoclastsCO—culturesystem.METHODS:(1)0Bwcreseparatedfromtheskullof SDratsaged1dandOCfromthethighboneandshinboneofSDratsaged5d.theplaneOBandOCCO—culturesystemw明estab— hshed,inwhichtheceilsineachculturesystemdidnotintermix,buttheculturemediumcommunicated,andBBC—containing ~IMIngroupandcontrolgroupWel~concludedintheexperiment.(2)FemaleSDratsaged10monthsweredividedintoBBCgroup andcontrolgrouptoprepareBBC—containing~I3.UTIandcontrolsenm1.(3)TheTRAPsecretedbyOCWaSmeasuredbythediazol methodandtheabsorptionoflacunaformedbyOCwasobservedbymicroscopeinOBandOCCO—culturesystemtodeterminethe activityofOCwiththeeffectofBBC—containingSer1.UTI.(4)TheeffectofBBC—containing~13.1monOCapoptosisinOBandOC CO—culturesystemwasexaminedbyopticalmicroscopesandfluorescencemicroscopes.RESULTS:InOBandOCCO—culture system,theactivityofTRAP~cretedbyOCofBBC—containingselMmgroupwereobviouslylowerthanthatofcontrolgroupin48 h,72hand96h,theOCsurvivingnumberswereobviouslylowerthanthatofcontro