曲古菌素a对食管的癌ec细胞增殖和细胞周期的影响及其分子.doc

曲古菌素A对食管癌EC1细胞增殖和细胞周期的影响及其分子 ? mol/L inhibited the growth of EC1 cells. In 基础医学院病理生理教研室 河南省郑州市 450052 曲古菌素ATSA comparison with the control group, the percent- 作者贡献分布: 此课题由董子明、马俊芬及赵继敏设计; 研究 既是一种抗真菌 age of cells in G /G phase increased, while the 过程由马俊芬、江亚南、赵继敏及黄幼田操作完成; 数据分析 0 1 药物, 同时也是强 由马俊芬、赵继敏、赵明耀及董子明完成; 本论文写作由马俊 percentage of cells in S phase decreased 74.56% 大的组蛋白去乙 芬、赵继敏、赵明耀及董子明完成. 酰酶HDAC抑制 ± 1.34% vs 62.12 ± 0.52 and 14.52% ± 1.81% vs 通讯作者: 董子明, 教授, 450001, 河南省郑州市科学大道100号, 剂, 其生物学效应 郑州大学基础医学院病理生理学教研室. dongzm@//0. 27.50 ± 0.66, respectively; both P 0.05 in EC1 主要包括诱导细 收稿日期: 2009-09-29修回日期: 2009-11-14 cells treated with TSA at a concentration of 0.5 胞凋亡, 阻滞细胞 接受日期: 2009-11-16在线出版日期: 2009-12-08 mol/L. TSA induced G /G phase arrest in a μ 0 1 周期和诱导肿瘤 dose-dependent manner. Western blot analysis 细 胞 分 化不 同 WAF1/CIP1 结构的HDAC抑 indicated that the expression of p21 was Trichostatin A inhibits cell 制剂对不同的细 upregulated in EC1 cells treated with TSA at 胞系的作用及机 proliferation and induces concentrations of 0.5 or 1.0 μmol/L TSA for 24 制不同, 本实验探 cell cycle arrest in human hours both P 0.05. 讨TSA对食管癌 esophageal carcinoma cell line EC1细胞增殖和 细胞周期的影响. TSA is able to inhibit cell EC1 CONCLUSION: proliferation and induce G /G arrest in hu- 0 1 Jun-Fen Ma, Ya-Nan Jiang, Ji-Min Zhao, man esophageal carcinoma cell line EC1 partly WAF1/CIP1 You-Tian Huang, Ming-Yao Zhao, Zi-Ming Dong through upregulation of the p21Key Words: Trichostatin A; Cell cycle; Esophageal Jun-Fen Ma, Ya-Nan Jiang, Ji-Min Zhao, You-Tian WAF1/CIP1 Department of Huang, Ming-Yao Zhao, Zi-Ming Dong, carcinoma; p21 Pathophysiology, College of Basic Medical Sciences, Zheng- zhou University, Zhengzhou 450052, Henan Province, China Ma JF, Jiang YN, Zhao JM, Huang YT, Zhao MY, Dong Correspondence to: Professor Zi-Ming Dong, Department ZM. Trichostatin A inhibits cell proliferation and induces of Pathophysiology, College of Basic Medical Sciences, cell cycle arrest in hu