小儿咳喘灵颗粒中的盐酸麻黄碱含量测定的种hplc法比较.doc

小儿咳喘灵颗粒中盐酸麻黄碱含量测定的2种HPLC法比较 小儿咳喘灵颗粒中盐酸麻黄碱含量测定的2种HPLC法比较 洪美华,,卓小萍,何世柯,宋增炫,周本宏(1.武汉大学药学院,武汉市430072;2.广东汕尾市药品检 验所,汕尾市516600) 中图分类号R283.627;R927.2文献标识码A文章编号1001—0408(2008)27—2122—03 摘要目的:比较测定小儿咳喘灵颗粒中盐酸麻黄碱含量的2种高效液相色谱(HPLC)法.方法:(1)法色谱条件:色谱柱为 PhenomenexCl8(250mm×4.6mm,5m),流动相为甲醇一水(1:1),检测波长为254nm;(2)法色谱条件:色谱拄为Phe— nomenexC18(250mm×4.6mm,5m),流动相为乙腈一磷酸溶液(内含0.02mol?L磷酸二氢钾,0.01mol?L磷酸)=5: 95,检测波长为210nm.结果:(1)法盐酸麻黄碱的检测浓度在0.08--40Fg?mL范围内与峰面积积分值呈良好的线性关系 (r=0.9999),平均回收率为101.72%,RSD=2.77%;(2)法盐酸麻黄碱的检测浓度在O.4～400弘g?mL.范围内与峰面积积 分值呈良好的线性关系(r=1.0000),平均回收率为99.60%,RSD=2.91%.结论:2种HPLC法结果均准确,专属性,重现性 均好,但(1)法提取更完全,朱使用有机溶剂,操作性更强. 关键词高效液相色谱法;小儿咳喘灵颗粒;盐酸麻黄碱;比较分析 DeterminationofEphedrineHydrochlorideinXiaOerkechuanlingGranulesbyTwoDifferentHPLCMeth- ods:AContrastiveAnalysis HONGMei—hua,ZHOUBen—hong(CollegeofPharmacy,WuhanUniversity,Wuhan430072,China) HONGMei—hua,ZHUOXiao—ping,HEShi—ke,SONGZeng—xuan(Guangd.ngShanweiInstituteforDrugCo— ntrol,Shanwei516600,China) ABSTRACTOBJECTIVE:TodeterminethecontentofEphedrinehydrochlorideinXiaoerkechuanlinggranulesbytwo HPLCmethods.METHoDS:ThechromatographicconditionsforthefirstHPLCmethodwereasfollows:thephenomenexC18 column(250mm×4.6nlm,5”m)wasusedwithmethanol—water(1:1)asmobilephaseatadetectionwavelengthof254nm; thechromatographicconditionsforthesecondHPLCmethodwereasfollows:thephenomenexC18column(250mm×4.6mm,5 Fm)wasusedwithacetonitrile—phosphoricacidsolution(contain0.O2mol.Lpotassiumdihydrogenphosphateand0.01 tool’Lphosphoricacid)(5:95)asmobilephaseatadetectionwavelengthof210nm.RESULTS:ByHPLCmethodone,the linearrangeofEphedrinehydrochloridewas0.08gmL～4Og’mL(1r0.9999)withtheaveragerecoveryat101.72% andtheRSDat2.77%;bythesecondHPLCmethod,thelinearrangeofEphedrinehydrochloridewas0.4Fg?mL～400 Fg?rnL(r1.0000)withtheaveragerecoveryat99.60%andtheRSDat2.91%.CONCLUSION:BothofthetwoHPLC methodsareaccurate,specificandreproducible,butbythefirstHPLCmethod,theextractionwasmorecompleteandmore operable,andnoorganicsolventwasused. KEYWORDSHPLC;Xiaoerkechuanlinggranules;Ep