大鼠与小鼠辨证论治标准的建立与用途.docVIP

大鼠和小鼠辨证论治标准的建立和用途 作者：方肇勤, 潘志强, 卢文丽, 刘小美, 管冬元, 梁超 【摘要】 大鼠和小鼠辨证论治方法和标准是具有中医药行业特色的方法和标准，其建立有助于满足中医药辨证论治研发的需要。本文介绍了建立大鼠和小鼠辨证论治标准所开展的系列研发工作，发现：（1）大鼠和小鼠可以用于模拟中医临床的诊法和辨证，可以采集到大量的四诊信息，满足辨证论治的需要；（2）利用大鼠和小鼠可以实现诊法和辨证的标准化、计量化，所建立的方法便于实验操作与普及；（3）正常大鼠和小鼠存在体质和证候的差异，常见疾病大鼠和小鼠会自发形成证候，发生证候的兼夹和演变，与人类近似；（4）证候存在复杂的物质基础，涉及神经内分泌免疫 网络 各组织基因表达与剪接的差异；（5）利用大鼠和小鼠可以开展个体化辨证论治及计量化疗效比较与评价。该方法和标准的主要用途包括：（1）证候模型动物的证候属性鉴定；（2）中医基础学科的研究，如藏象理论、证候的物质基础、辨证论治方法和作用机制、中医诊断学等研究；（3）中医临床学科的研究，如辨证论 治疗 效的比较与评价、辨证论治方案的优化、防治未病的研究；（4）中药学科的研究，如中药药性的研究、中药和方剂的药理研究等。 【关键词】 大鼠; 小鼠; 疾病; 证候; 四诊客观化; 辨证; 辨证论治; 药性 　　Abstract: Methodology of syndrome differentiation and syndromebased treatment in rats and mice has professional characteristics and caters to the research and development of traditional Chinese medicine (TCM). In this paper, the authors introduced thEir systematic research in five aspects. 1) Rats and mice can be used to simulate TCM clinical practice. Diagnosis and syndrome differentiation can be done to the rats and mice, and information collected by the four diagnostic methods from the experimental animals meets the requirements of treatment based on syndrome differentiation. 2) Standardized and quantified four diagnostic methods and syndrome differentiation for rats and mice can be established, and are operational and applicable for general use. 3) There exists constitution and syndrome diversity in normal rats and mice. A spontaneous syndrome can develop in diseased rats and mice, and it can be accompanied by or even change to another syndrome, similar to that in human bEIngs. 4) There is a complicated material base for syndromes inferred from the different gene expressions and splices in neuroendocrineimmune network. 5) Individualized treatment based on syndrome differentiation, as well as quantified evaluation and comparison of the treatment efficacy can be done in the rat and mouse models of syndromes. The established methodology and criteria for syndrome differentiation and sy