分子和细胞免疫进展01.ppt

Atherosclerosis is an chronic inflammatory disease of arterial wall an accumulation of lipids infiltration of immunocytes, macrophages, T cells and mast cells vascular smooth muscle cells collagen. Atherosclerosis Atherosclerosis Treg Th17 Atherosclerosis ＋ － － Th17 The different CD4+ subsets have various roles in Atherosclerosis A ACS NC B C CD4 IL-17 SA ﹡﹡ ﹡﹡ ﹡﹡ ﹡﹡ 1. The circulating Th17 cells and IL-17 significantly increase in patients with ACS compared with normal control and stable angina NC： Normal control SA：stable angina pectoris ACS：Acute coronary syndrome ----- unstable angina acute myocardial infarction Th17 IL-17 Th17and IL-17 may be involved in atherosclerosis. 2. Establishment of two atherosclerotic mice models High fat diet to 16 weeks or 24 weeks ApoE-/- mice 8 weeks Rapid carotid model Standard model High fat diet For 6 weeks or 14 weeks plaque in Aortic root plaque in carotid ApoE-/- mice 8 weeks 3. The dynamic change of Th17 subset in spleen during the development of atherosclerosis (1) 0.5% C57 ApoE-/- C57 ApoE-/- 8 weeks IL-17 IFN-g C57 ApoE-/- 3. The dynamic change of Th17 subset in spleen during the development of atherosclerosis(2) Carotid C57 ApoE-/- C57 ApoE-/- 16 weeks 24 weeks 3.0% IL-17 IFN-g IL-17 IFN-g 4.4% Advanced plaque 4. The percentage of Th17 in spleen has a positive correlation to size of plaque 8 weeks 16 weeks 24 weeks CD4+IL-17+(%) Plaque area( ?m2) Th17 5. Expression of IL-17 and its transcriptional factor ROR-?t in arterial walls during formation of plaque Established plaque advanced plaque ROR-?t IL-17 b-actin No plaque IL-17 macrophage CD4+ T cells 5. IL-17 is expressed in both CD4+ T cells and Macrophage Th17 and IL-17 are involved in the atherosclerosis Neutrolizing anti-IL-17 antibody or exogenous IL-17 was injected into ApoE-/-mice to confirm causative role High fat diet + Neutrolizing