绿豆幼苗对汞胁迫生理响应.docVIP

绿豆幼苗对汞胁迫生理响应 　　摘要：用沙培法探究绿豆幼苗在生长过程中受到Hg2+胁迫后的生理响应。试验结果表明，当Hg2+浓度小于1 mg/L，根活力有所增强，而随Hg2+胁迫浓度增加，根活力下降；当Hg2+浓度为80 mg/L时，其活力为对照组的21%，根老化加速。植株叶绿素含量随Hg2+浓度的增加而呈现下降趋势，Hg2+浓度大于10 mg/L时，对叶绿素含量的影响尤为明显；绿豆幼苗体内SOD、CAT活力对Hg2+胁迫反应相似，当Hg2+浓度为1～10 mg/L时，其SOD、CAT活力呈现上升趋势并高于对照组；之后则呈现下降趋势，并低于对照组。当Hg2+浓度为80 mg/L时，SOD、CAT活力分别为对照组的36%和32%。绿豆幼苗体内脯氨酸和丙二醛的含量随Hg2+浓度增加而增加。 　　关键词：汞离子；胁迫；绿豆；幼苗；生理响应 　　中图分类号：S522 文献标识码：A 文章编号：0439-8114（2012）21-4736-03 　　Physiological Response of Mung Bean Seedling to Hg2+ Stress 　　LIU Zhi-wei，WANG Xiao-ping，MAO Shan-guo 　　（Department of Biology Chemistry and Environmental Engineering，Nanjing Xiaozhuang College，Nanjing 211171，China） 　　Abstract： The physiological response of mung bean seedling under Hg2+ stress was studied by sand culture method. The results showed that when concentration of Hg2+ was lower than 1 mg/L， the root vigor was enhanced. As the stress concentration of Hg2+ increased， root the vigor was weakened； when Hg2+ concentration was 80 mg/L， the root vigor was 21% of that in control； and the aging of root was speed up. The chlorophyll content decreased with the increase of Hg2+ concentration； the impact was especially strong when Hg2+ concentration was higher than 10 mg/L. The SOD and CAT activity reacted similarly to Hg2+ stress. SOD and CAT activity increased and was higher than that of control when Hg2+ concentration was 1～10 mg/L， then decreased with the increase of Hg2+ concentration and was lower than that of control. When Hg2+ concentration was 80 mg/L， SOD and CAT activity was 36% and 32% of the control respectively. The content of Pro and MDA increased with the increasing of Hg2+ concentration. 　　Key words： Hg2+； stress； mung bean（Phaseolus vadiatus L.）； seedling； physiological response 　　汞元素在工业生产上被广泛应用，处理不彻底的废水、废渣中的汞进入环境后，大部分存在于土壤的表面，对植物有着??接的伤害[1，2]，并由生物链危及人类健康[3]。绿豆（Phaseolus vadiatus L.）具有良好的食用、药用价值，是被广泛种植的农作物之一。随着人们生活水平的提高，人们对其需求量也逐步增加，同时其品质也备受关注。对绿豆幼苗生长受到重金属胁迫的生理响应的研究不仅有助于了解植物耐重金属胁迫的生理生化机制，而且能为农田绿豆种植重金属污染的监测和综合治理方面提供理论依据。 　　1 材料与方法