Confined Polmers Big World in a Small Space密闭的聚合物大世界在一个小空间.pptVIP

Confined Polymers: unity of thought * in the test tube * in polymer brushes * in mesoscopic channels Adrian Parsegian and many friends: Sergey Bezrukov, Joel Cohen, Per Lyngs Hansen, Rudi Podgornik, et al., et al. In test tubevs. grafted, between bilayersvs. inside a pore  Today consider full data sets for two chemically different systems Transform variables, collapse all data for each polymer type How do the data collapse? A happy consequence of adding Cross at places different from Cp = Cp* Extract ?’s; one “universal” curve Width of crossover region What have we done? [Cohen, Podgornik, Parsegian 2007] Test tubevs. grafted, between bilayersvs. inside a pore  Schematic of force measurementbetween PEG lipids Multilayer Squeeze with Posmotic ofPEG in bathing solution! PEGylated, close-up of brushes grafted 5000 MW PEG polymers Posmotic vs. separation De Gennes, Adv. Coll. Interf. Sci. 27:189 (1987)Two types of grafted surfaces: a) low grafting – the distance between heads D is larger than the coil size RF, (“mushroom”)b)high grafting density D RF, (“brush”) Test tubevs. grafted, between bilayersvs. inside a pore  I vs. V Little guys easy in Big guy tough push,otherwise no go ?-Hemolysin Channel conductance vs. [PEG]Bezrukov, Krasilnikov, Kasianowicz PEG 3400 pushed into ?-Hemolysin channel How big (small) to fill a channel without any leftover?(S. Bezrukov, personal communication, last Thursday) High-concentration regime as “ideal” limit (des Cloizeaux) Pressure of bath == * small correlation length, * drive into channel of similar dimensionMovement of molecules in cells Test-tubes,  brushes, pores? new unity in thinking about * large molecules at high concentration  *the interior of cells and pores Merci! Add names Add picture of radius of gyration on top of pore diameter * Laboratory of Physical and Structural Biology, National Institute of Child Health and Human Development, National In