分子病毒 8病毒载体.pptVIP

* 韩 晓 Methods of gene delivery Viral Vectors: Adenovirus Retrovirus Lentivirus Adeno-associated virus (AAV) Herpes simplex virus (HSV) Non-viral vector based Naked DNA (plasmid DNA): injection or genegun Liposomes (cationic lipids): mix with genes Ex-vivo In vivo Why use viral vectors Virus are obligate intracellular parasites Very efficient at transferring viral DNA into host cells Specific target cells: depending on the viral attachment proteins (capsid or glycoproteins) Gene replacement: non-essential genes of virus are deleted and exogenous genes are inserted Generation of viral vector