白花败酱草提取物对小鼠前列腺增生实验研究.docVIP

白花败酱草提取物对小鼠前列腺增生实验研究 　　[摘要] 目的 研究白花败酱草提取物（PVJE）对小鼠前列腺增生的抑制作用。 方法 小鼠随机分为对照组、模型组、普乐安组、小剂量PVJE组、大剂量PVJE组。对照组皮下注射花生油溶液0.1 ml/只，其余各组均皮下注射丙酸睾酮溶液5 mg/（kg·d），同时普乐安组给予5 g/（kg·d）的普乐安溶液灌胃，小剂量PVJE组、大剂量PVJE组分别给予0.02、0.04 g/（kg·d）的白花败酱草提取物溶液灌胃，共给药12 d。研究小鼠前列腺湿重、前列腺指数、性激素水平和光镜下前列腺形态学的变化。 结果 与模型组比较，PVJE能明显抑制由丙酸睾丸酮诱发的小鼠前列腺增生，小鼠血清睾丸酮（T）、和雌二醇（E2）含量明显降低。 结论 PVJE对丙酸睾丸酮所致小鼠前列腺增生具有显著的拮抗作用。 　　[关键词] 白花败酱草；提取物；前列腺增生；小鼠 　　[中图分类号] R282.71 [文献标识码] A [文章编号] 1674-4721（2013）11（c）-0033-02 　　Experimental study of Patrina Villosa Juss. extractive on benign prostatic hyperplasia model of mice 　　GUO Xiao-qiu1 XU Jie1 ZENG Ling1 ZHANG Wen-ran1 ZENG Jing2 　　1.Department of Pharmacy， Hospital of Traditional Chinese Medicine in Jianxi Province， Nanchang 330006；2. Pharmacy College of Gannan Medical College in Jianxi Province， Ganzhou 341000，China 　　[Abstract] Objective To study inhibiting effect of Patrina Villosa Juss extractive（PVJE） on benign prostatic hyperplasia of mice. Methods Mice were divided into five groups including positive control group， model group， Pulean tablets treating group， low dose PVJE group and high dose PVJE group. The mice in control group were injected subcutaneously with peanut oil solution（0.1 ml）， while in other groups testosterone propionate solution [5 mg/（kg·d）] was employed instead of the oil. Meanwhile Pulean group was given gavage with Pulean 5 g/（kg·d）， and low dose PVJE group and high dose PVJE group were given PVJE gavage[0.02， 0.04 g/（kg·d）] for 12 consecutive days. Wet weight of prostate， prostate health index， sex hormone levels and prostate morphological changes under light microscope were compared among different groups. Results Compared with model group， PVJE can apparently inhibit prostatic hyperplasia in mice induced by testosterone propionate， reduce the serum testosterone（T）， estrodiol（E2） contents. Conclusion PVJE have significant inhibiting effect on benign prostatic hyperplasia induced by testosterone propionate in mice. 　　[Key words] Patrina Vill