微透析技术在植物生理生化研究中应用.docVIP

微透析技术在植物生理生化研究中应用 　　摘要：微透析是一种可以应用于生物体体内和体外的样品处理技术。可以与多种分析测试仪器耦合，对细胞外空间或其他水溶液环境中随时间、浓度发生变化的生理活性物质提供实时动态的监测信息。其突出优点是在不影响生物体正常生长的情况下可以准确地给出被分析物随时间的变化情况，而且可以对目标生物体进行各种处理，以得出被分析物受外界环境影响下的即时变化情况，而且微透析的样品在用高精密仪器分析时，无需进行样品前处理，可真正做到实时、在线、廉价的跟踪检测研究。阐述了微透析在植物体上的应用，微透析技术应用在动物体，主要取样是细胞间质，在植物体上可以检测植物质外体各种物质的变化，克服以往研究质外体的瓶颈，还包括了微透析技术与GC、HPLC、CE等分析仪器的耦合使用研究现状。 　　关键词：微透析；植物；质外体；HPLC；GC；CE 　　中图分类号：O652 文献标识码：A 文章编号：0439-8114（2013）12-2733-04 　　Application of Microdialysis Technique in Physiology and Biochemistry of Plant Research 　　MA Jin-long1，2b，JIANG Guo-bin2a，YAO Shan-jing1，JIN Hua2a，DENG Shao-li2a 　　（1.Department of Chemical and Biological Engineering， Zhejiang University，Hangzhou 310027，China； 2a. Environment and Resources College； 2b.Life Science College， Dalian Nationalities University， Dalian 116600，Liaoning，China） 　　Abstract： Microdialysis is a sampling technique that can be employed to monitor biological events both in vivo and in vitro， it can be coupled with a variety of analytical instruments， can provide monitoring information for biological active substances changed with time and concentration in other aqueous environment or outside the cells dynamically in realtime. It is advantageous in fast， selective and sensitive analysis while preserving temporal information without affecting the growth of organisms. At the same time， the changes of analyte can be detected immediately in external environment. Furthermore， microdialysis samples without pretreatment， which are coupled with high-precise analytical systems， will realize truly real-time， online and cheap tracking detection.Although microdialysis sampling focusing on intercellular matrix has been applied in animals and human， it has not been extensively employed to detect various material changes in plant apoplast. So microdialysis sampling technique can overcome the bottleneck of previous research on plants. An overview of microdialysis system about principle， probe， membrane and parameters， furthermore sampli