em相关.doc

The vaccination potential of EMY162 antigen against Echinococcus multilocularis infection Hirokazu Kouguchia, Jun Matsumotob, Yoshinobu Katoha, Yuzaburo Okub, Tomohiro Suzukia and Kinpei Yagia aHokkaido Institute of Public Health, N19, W12, Kita-Ku, Sapporo 060-0819, Japan bLaboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan Received 4 September 2007.? Available online 18 September 2007. Abstract Alveolar echinococcosis is caused by infection with the larval stage of Echinococcus multilocularis. We recently identified a cDNA clone, designated as emy162, that encodes a putative secreted protein. EMY162 shares structural features with the EM95 antigen, which is a host-protective antigen. The amino acid sequence of EMY162 shows 31.4% identity to EM95 whereas these antigens are distinguishable with respect to their predicted secondary structure and antigenicity on Western blot analysis. RT-PCR analysis revealed that the gene expression of emy162 was significantly higher than that of em95 at each life-cycle stage. Recombinant EMY162 antigen induced a significant level of host-protection (74.3%) in experimental infection with E. multilocularis eggs in mice. Notably, recombinant EMY162 antigen showed significant reactivity to the sera from alveolar echinococcosis patients. These results may help in the development of a practical vaccine to reduce the level of alveolar echinococcosis in humans. Keywords: Echinococcus multilocularis; Alveolar echinococcosis; EMY162; Vaccine; EM95; Host protective antigen Article Outline Materials and methods Results and discussion Sequence analysis of EMY162 and EM95 RT-PCR analysis of emy162 and em95 Expression and purification of recombinant vaccine antigens Vaccine trial of recombinant EMY162 and EM95 Immunogenicity of EMY162 and EM95 Acknowledgements References Alveolar echinococcosis (AE) in humans is a zoonosis caused by infection wit