Microtubule Tracking - Yale University微管跟踪-耶鲁大学.pptVIP

Tracking the Motion of the Outer Ends of Microtubules Stathis Hadjidemetriou, Derek Toomre, James S. Duncan Yale University, School of Medicine, New Haven, CT 06520 Microtubule (MT) Assembly Why are Microtubule Dynamics Interesting? Importance in Cell Pathology Anticancer drugs (e.g. Taxol?, Taxotere?) inhibit polymerization of MTs to retard: Cancer growth through cell division Cancer spread through cell migration Objective Previous Work in Segmentation of Biomedical Curvilinear Structures Previous Work in Motion Tracking of Biomedical Structures Confocal Microscopy Pinhole microscopy, 2D or 3D Resolution: x-y≈200 nm/pixel, z≈500 nm/pixel, weff≈2-3 pixels Laser causes photobleaching Preprocessing Filtering: Largest eigenvalue of matrix of 2nd derivatives MT Segmentation in Initial Frame Cumulative Cost Over Valid Neighborhood Centerline Component Extraction of Curve Segment Extraction of First MT Segment Extraction of Subsequent MT Segments Evaluation of Curve Segment Microtubule 2D Segmentation Examples of Segmentations of 2D MT MT Extrapolation in Average Frame Compute average image over time: MT Extrapolation in Average Frame Thresholding of Data Streamline Passing from Microtubule Examples of Streamlines Through Microtubules Compute MT End Feature MT End Point Trajectory Outline of Motion Tracking Algorithm Phantom Sequence for Noise Examples 1 of Real Sequence Example 1: Ground Truth Example 2 of Real Sequence Example 2: Ground Truth Example 4 of Real Sequence Summary Discussion End Confocal vs Conventional Microscopy Quantification of Microtubule Motion Quantification of Microtubule End Motion Examples of Motion Statistics Example 2: Statistics Green: Ground truth Red: Algorithm Error in MTs tracked=17/19 Error in tracking=2.6 pixels Example 3 of Real Sequence Size=136x112x100, Time=5 min 7 sec Size=350x262x36, Time=3 min 25 sec, Error in MTs tracked=17/19 Error in tracking=2.6 pixels Green: Ground truth Red: Algorithm Preprocessing MT segmenta