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毕 业 设 计（论 文） 不同植物生长调节物质对 甘蓝下胚轴愈伤组织诱导的影响 专 业 学生姓名 班 级 学 号 指导教师 完成日期 不同植物生长调节物质对 甘蓝下胚轴愈伤组织诱导的影响 摘要：以MS培养基为基础，添加植物生长调节物质生长素和细胞分裂素，对甘蓝下胚轴进行愈伤组织培养，分析生长素和细胞分裂素在愈伤组织培养过程中，对愈伤组织的诱导和生长的影响，为植物愈伤组织培养提供参考。 关键词：生长素；细胞分裂素；愈伤组织；组织培养 Different Plant Growth Regulators on Brassica hypocotyl callus induction Biotechnology 06 (2) classes Zhang Xin Abstract：MS-based medium, add the plant growth regulators auxin and cytokinin, on the cabbage hypocotyl callus tissue culture for analysis of auxin and cytokinin in the process of callus tissue culture of callus Induction and growth of, for callus tissue culture plants for reference. Key words: auxin；cytokinin； callus；tissue culture 目　　　　录 1 引言·····································································3 2 材料与方法·······························································3 2.1外植体的获取·····························································3 2.2植物生长调节物质的选择···················································3 2.3培养基配制·······························································4 2.4诱导愈伤组织培养·························································4 2.5实验观察·································································4 3 结果和分析······························································5 3.1不同激素组合和配比对愈伤组织发生的影响···································5 3.2不同激素组合和配比对愈伤组织根分化的影响·································5 4 小结····································································5 参考文献····································································7 致谢··················································································8 不同植物生长调节物质对 甘蓝下胚轴愈伤组织诱导的影响 Lll 学院 摘要：以MS培养基为基础，添加植物生长调节物质生长素和细胞分裂素，对甘蓝下胚轴进行愈伤组织培养，分析生长素和细胞分裂素在愈伤组织培养过程中，对愈伤组织的诱导和生长的影响，为植物愈伤组织培养提供参考。 关键词：生长素；细胞分裂素；愈伤组织；组织培养 Plant Growth Regulators on Brassica hypocotyl callus induction Abstract：MS-based me