EASYspin 植物RNA快速提取试剂盒.doc

EASYspin Plus 植物RNA快速提取试剂盒 ?本公司独家推出EASYspin无苯酚、氯仿RNA快速提取技术基础上，又独家研发成功基因组DNA清除柱技术可以有效清除gDNA残留，得到的RNA一般不需要DNase消化，可用于反转录PCR、荧光定量PCR等实验。独特的裂解液迅速裂解细胞和灭活细胞RNA酶，植物RNA助提剂PLANTaid帮助结合多糖多酚并通过离心去除，然后裂解混合物用乙醇调节RNA结合吸附到基因组DNA清除柱，基因组DNA清除柱子同时吸附清除残留的DNA, 然后RNA被选择性洗脱滤过。滤过的RNA用乙醇调节结合条件后，RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜， 再通过一系列快速的漂洗－离心的步骤, 去蛋白液和漂洗液将细胞代谢物，蛋白等杂质去除， 最后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。 产品特点： 使用EASYspin/EASYspin Plus试剂盒已经有大量文章发表，请联系我们索取发表的文章和包括北京林业大学，北京农学院，中国农业大学，农林科学院果树研究所，林业所，中国农科院等大学和研究院所大量使用案例。 1.?????Isolation, characterisation and phylogenetic analysis of resistance gene??? analogues in a wild species of peach (Prunus kansuensis).Canadian Journal of Plant Science,??? 2011, 91(6): 961-970 2.?????樱桃花、叶、颚等各部位：Over-expression of the PaAP1 gene from sweet cherry (Prunus avium??? L.)??? causes early floweri.Journal of Plant Physiology, 2012,Available online 1 December??? 2012 3.?????洋葱根、茎、蕾、叶、雌雄蕊等各部位：Cloning and Expression Analysis of A Putative B Class MADS-box??? Gene of AcPI in Onion. Scientia Agricultura Sinica, 2012, 45(23):4759-4769 4.?????芜菁：Isolation and Functional Characterisation of the Genes Encoding Δ8-Sphingolipid?????? Desaturase from Brassica rapa. Journal of Genetics and Genomics Volume 39, Issue 1, January??? 2012,??? Pages 47–59 5.?????芜??菁?1?：EXPRESSION, DIVERGENCE AND EVOLUTION OF THE CALEOSIN GENE FAMILY IN BRASSICA?RAPA. Arch. Biol. Sci.,?Belgrade, 65 (3), 863-876, 2013 DOI:10.2298/ABS1303863H 6.?????番茄叶：Effect of Low Temperature Stress on the Expression of ProDH Gene and the Activities of the Proline Dehydrogenase in Leaves of Tomato Seedling. Chinese Agricultural Science??Bulletin 2012,28(10):132-135 7.?????栀子叶：Isolation of High Quality Total RNA from Gardenia jasminoides Eills.Chinese Agricultural??? Science Bulletin.2012, 28(27):194-198 8.?????油桐果实：Cui Qinqin, Han Xiaojiao, Chen Yicun, Zhan Zhiyong, Lin Liyuan, Wang Yangdong.???? Isolation and Expression Characteristics of Biotin Carboxyl Carrier Protein Coding Gene（VfBCCP)? from Vernicia fordii.SCIENT