利用蔗渣半纖維素水解液以固定化酵母菌酵生產木糖醇 一前言.docVIP

利用蔗渣半纖維素水解液以固定化 酵母菌發酵生產木糖醇 廖文城 劉富雄 陳觀彬 國立雲林科技大學化學工程系 Pretreatment of Sugar Cane Bagasse Hemicellulose Hydrolyzate for xylilol Production by Immobilized Candida subtropicalis W. C. Liaw, F. S. Lin, K. B. Chen Department of Chemical Engineering National Yunlin University of Science and TechnologyYunlin, Taiwan, R.O.C 摘 要 本研究是利用親水性壓克力樹脂，2-hydroxyethyl methacrylate(HEMA)為主成份，再配合交聯劑Polyethylene glycoldiacylate (molecular weight=1000)，並加入1%的Benzoin isopropyl ether(光增感劑)及酵母菌-Candida sp.以UV光共聚合後，則形成機構強度及含水率佳的水膠薄膜。而所使用的原料則以農業副產物-蔗渣，其經加酸水解後，所得到的半纖維素水解液，含有的抑制物會抑制酵母菌的活性，本實驗便利用活性碳，有機溶劑萃取及陽離子交換樹脂等三種方式處理後，結果顯示以陽離子交換樹脂處理過的水解液，將所製得的固定化酵母菌膜發酵後，所得的最佳轉化率在基質濃度為7%，轉速為150rpm，菌體濃度為5.75mg/g,film，可得到60%。對於所進行批式持久性測試。可長達到1~2個月。其轉化率可達到40%以上。 關鍵詞：木糖醇、木糖、固定化酵母菌、壓克力樹脂 ______________________________________________________ 廖文城 雲林科技大學化學工程系副教授 Tel: (05)5342601轉4618 E-mail::liawwc@pine.yuntech.edu.tw 陳觀彬 雲林科技大學工程博士班博一學生 ABSTRACT This study utilized 2-hydroxyethyl methacrylate(HEMA), a hydrophilic acrylic resin, as the main ingredient for the formation of a hydrogel film having high water content and mechanical strength. The other ingredients used were polythylene glycol diacrylate(molecular weight=1000)which was a cross-linking agent and 1% of Benzoin isopropyl ether(a photosemsitizer)and yeast cells of Candida sp.. Polymerization was initiated with UV light.The raw material for fermentation was an agricultural biproduct-bagasse,after acid hydrolysis,it yielded hemicellulose solution. Since this solution contained susbstances that inhibited the activity of yeast cell,we used three yreatment methods is activated charcol adsorption, organic solvent extraction and cationic exchange resin treatment to remove the unwanted substanced. Fermentation was them carried out in this solution by adding the immobilized yeast cell film and adjusting the substrate concentration to 7%, cell concentration to 5.75 mg/g,film. The yield was 60% when the rotation speed was 150rpm. The immobilized cells were then treat