tgf-β1 mmp-2和timp-2在煤工尘肺病中表达及意义-expression and significance of tgf - β 1 mmp - 2 and timp - 2 in pneumoconiosis of coal workers.docx

tends to increase, the ratio of MMP-2/TIMP-2mRNA was increased in category II, the difference was statistically significant (P0.05), the ratio of fluid MMP-2/TIMP-2mRNA in category III was lower than category II, the difference was statistically significant (P0.05), Correlation analysis showed that serum TGF-β1, MMP-2 and TIMP-2, respectively, and the whole lung lavage TGF-β1, MMP-2, TIMP-2 levels were positively correlated (P0.05), there was no correlation between levels of whole lung lavage fluid TGF-β1 protein expression and TGF-β1mRNA, TIMP-2 protein expression and TIMP-2mRNA, MMP-2 protein expression and MMP-2mRNA, the difference was not statistically significant (P0.05). Conclusion:The higher expression of serum TGF-β1, MMP-2, TIMP-2 might be related to the occurrence and development of CWP. With the change of lavage fluid TGF-β1, TIMP-2 protein and gene expression levels, and correlation of the serum and lavage TGF-β1, TIMP-2, suggesting that the serum TGF-β1 and TIMP-2 could indirectly reflect the severity of the disease. The positive correlation was higher among Serum TGF-β1, MMP-2, TIMP-2, TGF-β1, MMP-2 and TIMP-2 play a key role in the occurrence and development of CWP. Key words: coal workers pneumoconiosis, transforming growth factor-β1, matrix metalloproteinase -2, tissue inhibitor of matrix metalloproteinase -2, extracellular matrix  主要符号表 缩略语 WLL 英文全称 Whole lung lavage 中文全称 大容量全肺灌洗 TGF-β MMPs TIMPs MT-MMP Transforming Growth Factor-β Matrix Metalloproteinases Inhibitor of Matrix Metalloproteinases Membrane-type matrix degrading enzymes 转化生长因子-β 基质金属蛋白酶 基质金属蛋白酶组织抑制因子 膜型基质降解酶 BALF Broncho-alveolar lavage fluid 支气管肺泡灌洗液 ECM ELISA Extracellular Matrix Enzyme Linked Immunosorbent Assay 细胞外基质 酶联免疫吸附测定 RT-PCR EB mRNA IL-6 Reverse transcription-PCR Ethidium bromide Messenger RNA Interleukin-6 逆转录聚合酶链式反应 溴化乙锭 信使 RNA 白细胞介素-6 学位论文独创性声明 本人声明，所呈交的学位论文系在导师指导下本文独立完成的研究成果。文中任何引 用他人的成果，均已做出明确标注或得到许可。论文内容未包含法律意义上已属于他人的 任何形式的研究成果，也不包含本人已用于其他学位申请的论文或成果。与我一同工作的