三参汤治疗糖尿病肾病的实验研究-中医基础理论专业论文.docxVIP

The experimental study of Sanshen Decoction on diabetic nephropathy treatmentAbstractObjective: To investigate the mechanism of preventing early diabetic nephropathy by Sanshen Decoction.Methods: The diabetic nephropathy of rat model is induced by modified tail vein injection the STZ, with the criterion of blood glucose and urinary albumin. An Bowei is the positive control group. Urinary Albumin, Urinary α1-microglobulin and Serum Cystatin C are indicators of renal dysfunction. In situ hybridization immunohistochemistry, ELISA and othermethods are used to detect gene expression： plasma and renal tissue ACEactivity and content, ATⅡ content. In order to reveal the mechanism of Sanshen Decoction in the treatment of early diabetic nephropathy, the effects of Sanshen Decoction on preservation of renal function and the restoration of kidney tissue are observed by analysing the renin-angiotensin system, vascular endothelial function.Results: Sanshen Decoction can reduce the body weight, blood glucose levels, MA, ET-1, Cystatin C, ATII, VEGF, etc. through the experiments index.Conclusion: The prevention and treatment of Sanshen Decoction on diabetic nephropathy rats are achieved by adjusting the rennin-angiotensin system. That is to say, the inhibition of renin and ACE leads to the reduction of angiotensin I and angiotensin II, thus the development of renin angiotensin system is blocked.Key words： Diabetic nephropathy; Sanshen Decoction; Angiotensin II;Endothelin-I; Cystatin C引言近年来我国糖尿病患病率已上升到5％左右，糖尿病肾病(Diabetic nephropathy, DN)是糖尿病慢性微血管病变并发症，是糖尿病最为严重的 并发症之一。DN一旦出现持续性蛋白尿，则肾功能持续性减退，是导致 终末期肾功能衰竭的重要原因。DN微量蛋白尿期持续时间长、病理过程 可逆，是防止病情发展为终末期肾病的关键阶段，因此，寻求有效的治 疗是全世界医学界面临的一个重要问题，而运用中西医结合治疗糖尿病 肾病，揭示中医治疗早期糖尿病肾病的理论依据和作用机制，对于减缓 慢性肾脏病的进展，促进祖国医学的壮大具有重要意义。现代医学对早期 DN 的治疗仍以控制血压、控制血糖、减少尿蛋白 为主，治疗药物首选血管紧张素转换酶抑制剂（Angiotensin-converting enzyme inhibitor, ACEI）类药物。祖国传统医学中，根据整体观念和辨证 论治理论，运用中药复方，在治疗 DN 中发挥了很大的作用。我们认为 肾虚络淤是 DN 的基本病机，益气活血养阴方——三参汤在临床治疗早 期 DN 已取得较为满意的疗效。现有研究已经证明三参汤对西药降糖药 控制欠佳的 2