Rb基因和PCNA在前列腺癌中表达意义及相关性分析.docVIP

Rb基因和PCNA在前列腺癌中表达的意义及相关性分析 作者：汤群辉　杨力军　王禾　陈宝琦　王鹏飞　武国军 【关键词】 前列腺肿瘤 　　关键词: 前列腺肿瘤;增殖细胞核抗原;视网膜母细胞瘤基因;免疫组织化学 　　摘 要:目的 探讨视网膜母细胞瘤易感基因（Rb）和增殖细胞核抗原（PCNA）在前列腺癌中表达的意义及其相互关系. 方法 采用免疫组织化学SABC法对36例前列腺癌标本Rb基因和PCNA进行检测，并应用图像分析仪及统计学方法对两者在不同病理分级及临床分期中的表达进行对比及相关分析. 结果 36例前列腺癌标本中Rb表达阳性17例，阳性率47.2%，其表达在前列腺癌的不同病理分级及临床分期之间有显著差异（Plt;0.05）;PCNA在标本中的表达强度在不同病理分级及临床分期之间有显著差异（Plt;0.05）;同时还发现Rb表达与PCNA表达存在负相关，即Rb阳性标本中PC-NA表达弱而Rb阴性标本中PCNA表达强，两者比较有显著性差异（Plt;0.05）. 结论 Rb基因参与了前列腺癌的发生、发展过程，在发病机制中可能涉及到Rb和PCNA调节通路的异常，检测Rb基因和PCNA有助于前列腺癌恶性程度的判定及预后. 　　Keywords:prostatic neoplasms;proliferating cell nuclear antigen;retinoblastoma genes;immunohis-tochemistry 　　Abstract:AIM To discuss the expression and correlation of retinoblastoma susceptibility gene and proliferating cell nu-clear antigen in the prostatic cancer.METHODS SABC　immunohistochemistry was used to determinate Rb gene and PCNA in36samples of the prostatic cancer.The image pat-tern analyzer and the statistics methods were used to do the comparative analysis of Rb gene and PCNA in the different pathological grading and clinical phases of the prostatic can┐cer.RESULTS immunohistochemistry staining was Rb pos-itive in17of36samples，rated47.2%.The expression of Rb had significant differences in different pathological grading and clinical phases（Plt;0.05）.The expression of PCNA also had significant differences in different pathological grading and clinical phases（Plt;0.05）.The expression of PCNA was weak in the Rb positive expression samples and was strong in the Rb negative expression samples.There was a significant difference between Rb and PCNA expression（Plt;0.05）.CONCLUSION Rb is involved in the occurrence and devel-opment of the prostatic cancer.As for pathological mecha-nism，it is related to the abnormality of Rb and PCNA regu-lating path.The determination of Rb and PCNA can be an in-dex for estimating the level of malignant and progression of the prostatic cancer. 　　0 引言 　　前列腺癌是威胁人类生命的恶性肿瘤之一，自20世纪70年代以来我国前列腺癌的发病率明显升高，对前列腺癌的诊治已日益