不同固着基 金属离子k cu2及l-dopa ne对香港巨牡蛎眼点幼虫固着变态影响的研究-effects of different fixation group metal ions k cu2 and l - dopa ne on fixation and metamorphosis of macrooyster eyespot larvae in hong kong.docxVIP

幼虫的最适变态诱导浓度也分别为10-5mmol/L、10-4mmol/L，其诱导最大变态率均在持续作用24h后再培育24h取得，其诱导变态率分别为65.36%、67.84%。关键词：香港巨牡蛎眼点幼虫固着基K+Cu2+L-DOPANE固着变态THERESEARCHOFSETTLEMENTANDMETAMORPHOSISOFCRASSOSTREAHONGKONGENSISEYESPOTSLARVALINDIFFERENTATTACHEDSUBSTRATE,METALION(K+,Cu2+),L-DOPAANDNEABSTRACTInthispaper,thesettlementandmetamorphosiseffectofCrassostreahongkongensiseyespotslarvaindifferentattachedsubstrate,twokindsofmetalion(K+,Cu2+)andL-3,4-dihydroxyphenylalanine(L-DOPA),norepinephrine(NE)wasstudied.Themainresultsofthisthesisaresummarizedasfollows：Thehighestofsettlementratewas49.85%intheblackpolyethylenefilm,followedbyblackplasticboard(19.33%),andthelowestwasredplasticboard(5.14%).AddingK+hadinducedthesettlementandmetamorphosisofeyespotslarva,whenadding13.42mmol/LK+disposing24h,larvalsettlementincreasingrateandmetamorphosisincreasingratereached42.39%and25.20%respectivelycomparedwiththecontrol.WhileaddingCu2+hadnoeffect,withtheincreaseofCu2+processingtimeandconcentration,themortalityrateofC.hongkongensiseyespotslarvaobviouslyenhancedwhichsuggestedthatexcessivelevelsofCu2+aretoxictoC.hongkongensiseyespotslarvae.AddingL-DOPAandNEhadeffecttoinducethemetamorphosisoflarval,butNEofoysterinductioneffectwasbetterthanL-DOPA.WhenaddingdifferentconcentrationofL-DOPAandNEcontinuousexposurethelarvalofC.hongkongensisafter12h,24hand48h,theoptimuminductionconcentrationofL-DOPAandNEwererespectively10-5mmol/Land10-4mmol/Lwithexposuring24h,thelarvalofC.hongkongensismetamorphosisratewere45.25%and57.14%,respectively;WhenaddingL-DOPAandNEexposurethelarvalofC.hongkongensis1hand24haftercultivatingfor24h,theinducedmaximummetamorphosisrateincontinuous24haftercultivatingfor24h,thelarvalofC.hongkongensis.metamorphosisratewere65.36%and67.84%,respectively.KEYWORDS:Crassostreahongkongensis;eyespotslarva;attachedsubstrate;K+;Cu2+;L-DOPA;NE;settlementandmetamorphosis目录第一章文献综述......................................................................................................................11、牡蛎固着变态的研究进展......................