不同时相力竭运动对大鼠心肌tnf-a icam-1及adamts-1的影响-effects of different exhaustive exercises on myocardial tnf - a icam - 1 and adam ts - 1 in rats.docxVIP

AbstractObjective: to figure out the pathological process and mechanism of exercise-induced myocardial micro-damage by discussing the time-course characters and rules of TNF-a, ICAM-1and ADAMTS-1 expression in myocardium.Method: 100 SD male rats were grouped into 3 groups: the control group (C, n=20), one-time exhaustive swimming group (O, n=40), 2-week repeated exhaustive swimming group (R, n=60).rats were sacrificed at 0, 6, 12, and 24h after exhaustive swimming. The expression of TNF-a, ICAM-1and ADAMTS-1 in myocardium were detected in the approach of immunofluorescence, immunochemistry and image analysis.Results:The expression of myocardium TNF-a showed a tendency of “increase-decrease” after both models of exhaustive swimming, and reached the summit 6 hours after swimming, greatly significant difference were found between all time-course groups and the control group（p0.01）.The myocardium TNF-a in R group expressed faster than that of O group, while itsexpression in O group was greater than that of R group（p0.05）. The expression of myocardium TNF-αat left ventricle was greater than that of inter-ventricular septum and right ventricle in O group（p0.05）, while it was greater at right ventricle than left ventricle and inter-ventricular septum in the R group（p0.05）.The expression of myocardium ICAM-1 also showed a tendency of “increase-decrease” after both models of exhaustive swimming, and reached the summit 6 hours after swimming, greatly significant difference were found between all time-course groups and the control group（p0.01） except for its expression at inter-ventricular septum and right ventricle in the 24h R group（P0.05）. the expression of myocardium ICAM-1 in R group was faster than that of O group, while its expression at left ventricle in O group was greater than in R group（P0.05）, and itsexpression at inter-ventricular septum and right ventricle in R group were greater than that in O group（P0.05，p0.01）.The expression of myocardium ADA