苯并a芘对a549细胞hif-1信号通路的影响-effect of benzo a pyrene on hif - 1 signaling pathway in a549 cells.docxVIP

目录中文摘要·······························································································（1）英文摘要·······························································································（2）论文题目·······························································································（3）前言···································································································（3）1实验材料····························································································（4）1.1细胞株·····························································································（4）1.2主要仪器··························································································（4）1.3主要试剂··························································································（5）1.4主要溶液及其配制··············································································（5）2实验方法····························································································（8）2.1人肺腺癌A549细胞的培养···································································（8）2.2MTT法检测B(a)P对A549细胞活力的影响·············································（8）2.3A549细胞药物处理·············································································（8）实时荧光定量PCR法检测CYP1B1、CYP1A1、CAⅨ、VEGFmRNA表达情况（9）Westernbloting法检测CYP1B1、CYP1A1、CAⅨ、VEGF的蛋白表达情况···（11）2.6免疫沉淀检测AhR和HIF-1α与ARNT/HIF-1β的结合程度··························（13）3结果································································································（14）3.1B(a)P对A549细胞活力的影响····························································（14）3.2B(a)P对A549细胞CYP1B1、CYP1A1、CAⅨ和VEGFmRNA表达的影响···（15）3.3B(a)P对A549细胞CYP1B1、CYP1A1、CAⅨ和VEGF的蛋白表达的影响···（15）3.4B(a)P对A549细胞AhR和HIF-1α与ARNT/HIF-1β结合程度的影响············（18）4讨论································································································（19）5结论································································································（20）参考文献···············