高直链淀粉玉米种质的类群研究及sbei、sbeiib基因分子标记的开发-study on groups of high amylose maize germplasm and development of molecular markers of sbei and sbe iib genes.docxVIP

高直链淀粉玉米种质的类群分析及 sbei、sbeIIb 基因分子标记的开发homology between the high amylose maize and sequences published in the database. There were 15 base-pair mutations in the sebi gene of GMES-0067, and 5 of them leaded to the amino acids mutations. While other high amylose maize materials showed no difference with the sequences published in the NCBI.5. SbeI from GEMS-0067 had 15 SNPs and all of them could be used as potential markers. The SNP that was positioned at the 1330th nucletiode on the 6th exon happened to be the enzymatic site of AluI which is very convenient to detect this SNP. SbeI-SNP amplified a PCR product of 906-bp from all 17 inbred lines used in this study. When completely digested by AluI, only the product from GEMS-0067 was not cut while the products from all other inbred lines were cut into two smaller fragments (617bp and 289bp), confirming the SbeI cDNA sequences of ae mutants. The co-dominat nature of SbeI -SNPwas reflected by digesting PCR product amplified from F1 heterogous Aeae genotype, which yielded three bands.Key words: Zea mays L; high amylose content; cluster analysis; molecular marker- assisted breeding4符号说明缩写符号英文全称中文名称3-PGAGlycerol-3-phosphate3-磷酸甘油ADPGAdenosine diphosphate glucose腺苷二磷酸葡萄糖AFLPAmplified fragment length polymorphism扩增片段长度多态性AGPaseAdenosine diphosphate glucosepyrophosphorylase腺苷二磷酸葡萄糖焦磷酸化酶CAPSCleaved amplified polymorphic sequences切割扩增长度多态性ESTExpressed sequence tagging表达序列标签 GBSSI/WxGranule-bound starch synthase束缚态淀粉合成酶 ISAIsoamylase异淀粉酶PICPolytnorphism information eontent多态信息量PCRPolymerase chain reaction聚合酶链式反应 RAPDRandom amplified polymorphic DNA随机扩增多态性 DNA RELimit dextrinase; R enzymeR 酶RFLPRestriction fragment length polymorphism限制性酶切片段多态性sbeIIb/aeStarch branching enzyme IIb淀粉分支酶 IIb 编码基因SDBEStarch debranching enzyme去分支酶SDSSodium dodecylsulfate十二烷基硫酸钠SNPSingle Nucleotide Polymorphisms单核苷酸多态性 SSRSimple sequence repeats简单序列重复(微卫星) SSSSoluble starch synthase可溶性淀粉合成酶 SUSSucrose Synthase蔗糖合成酶TPTriose phosphate磷酸丙糖UDPGuridine diphosp