磁性介质的研制及磁分离过程理论分析-development of magnetic medium and theoretical analysis of magnetic separation process.docx

中文摘要制备出以 Fe304 为磁性物质、聚乙烯醇为包埋材料、带有色素配 基的聚乙烯醇磁性亲和载体。制备过程中采用了两种不同的方法， 分别为水/油两相悬浮交联法和水相直接交联法。以溶菌酶和牛血清 白蛋白为目标蛋白，考察了载体对蛋白质的吸附行为。在甲基丙烯酸缩水甘油醋、二乙烯基苯和三聚异氧尿酸三烯丙醋 的聚合体系中添加以油酸为分散剂的磁流体，制备出刚性共聚物磁 性载体，经二乙肢修饰后，获得磁性大孔阴离子交换剂，对 BSA 的 吸附容量达 71.3 mg/ml，BSA 的有效扩散系数为 5.0x 10 13 m2/s 。通过实验证明了上述各种磁性载体具有良好的机械强度、化学稳 定性和重复使用性。载体的磁化过程符合 Langevin函数，属于超顺 磁体系。采用比油法对具有连续粒度分布的多分散颗粒的重力沉降过程 进行了研究，考察了 Beer 定律的适用范围。将 Kynch 单分散沉降模 型推广到多分散体系，对沉降过程进行了计算机模拟，并对不同的 阻滞沉降函数进行了比较。对磁性颗粒在磁场中的沉降过程进行了实验研究，并根据磁沉降 的特点建立了多分散磁沉降模型，对过程进行了理论分析。外加磁 场通过提供颗粒运动所需的驱动力及诱发颗粒聚集，可以显著缩短 固液分离时间。建立了用于模拟膨胀床流体力学行为的多分散一维两相流模型， 可以准确地预测床层膨胀高度随流体梳速变化而产生的动态响应， 以及吸附剂颗粒的浓度和粒度分布。分析了流体的非稳态流动及颗 粒的密度分布对模拟结果的影响。关键词:磁性载体，聚乙烯醇，蛋白质吸附，磁沉降，多分散颗粒， 膨胀床，数学模型ABSTRACTA magnetic support was produced by water/oil suspension crosslinking or aqueous-phase crosslinking of poly(vinyl alcohol) with the presence ofmagnetite coIIoids. Cibacron blue 3GA was immobilizedto the support as an affinity ligand. The adsorption behavior ofIysozymeand bovine serum albumin to the affinity support was studied.A poly(glycidyl methacrylate-triaIIyl isocyanurate- divinylbe皿ene) magnetic support was synthesized by adding oleic acid stabilized magnetite colloids to the reaction mixture. The support was derivatized wi也 die址lylamine to obtain a magnetic anion exchanger，which had a static capacity of 71.3 mg BSAlml wet support. The effective diffusivityofBSA in the support was 5.0x lO-13 m%_The above-mentioned m吨netic supports were mech缸让cally and chemicaIIy stable during recycling. Their magnetization curves could be weII described by the Langevin function， indicating thesuperparru口agnetic nature of these supports.The sedimentation of polydisperse agarose beads with broad particle size distributions was studied experimentally and theoreticaIIy. A Jight-extinction princìple was used to measure the solid concentration in the suspension at different time and settling distance咀. The procedurewas simulated with a polydispersesedimentation model developed 仕om Kynchs monodispersesed