共振光散射技术在蛋白质和核酸分析中的分析及应用-analysis and application of resonance light scattering technology in protein and nucleic acid analysis.docxVIP

秦惠：共振光散射技术在蛋白质和核酸分析中的研究及应用秦惠：共振光散射技术在蛋白质和核酸分析中的研究及应用IVIVHSA respectively, with a correlation coefficient of 0.9992, 0.9996 for BSA and 0.9995, 0.9993 for HSA, with the limits of determination of 8.3ng/mL for BSA and 7.4ng/mL for HSA. This method is simple, rapid and has been applied satisfactorily to determination of total proteins in human serum samples with satisfactory results.In the third chapter ， we studied the resonance light scattering spectralcharacteristic of eriochrome red B-Sodium dodecyl sulphonate-protein system, and the elementary reaction mechanism with absorption spectra method and a lot of condition experiments. Another new determination method of protein at nanogram level was established. In the Brittion-Robinson buffer of pH 2.87, RLS of the eriochrome red B-Sodium dodecyl sulphonate-protein system with the maximum scattering peak located at 563 nm. Under the optimum conditions, the enhanced RLS intensity was proportional to the concentration of proteins, over the range of 0.0-2.0mg/L and 2.0-8.0 mg/L for HSA and 0.0-3.5 mg/L and 3.5-12.5mg/L for BSA respectively, with a correlation coefficient of 0.9997, 0.9996 for HSA and 0.9992, 0.9998for BSA, with the limits of determination of 22.6ng/mL for BSA and 12.0ng/mL for HSA. This method is simple, rapid and has been applied satisfactorily to determination of total proteins in human serum and creamery samples with satisfactory results.In the fourth chapter ， we studied the resonance light scattering spectralcharacteristic of patent blue Ⅴ - Cetyltrimethyl ammonium bromide-nucleic acid system, and the elementary reaction mechanism with absorption spectra method and 1H NMR spectra method. Another new determination method of nucleic acid at nanogramlevel was established. In the Brittion-Robinson buffer of pH 9.9, RLS of the patent blueⅤ - Cetyltrimethyl ammonium bromide-nucleic acid system with the maximum scattering peak located at 345nm. Under the optimum conditions, the enhanced RLS