夏天无注射液对小鼠脑缺血和神经元保护作用的实验研究.docVIP

夏天无注射液对小鼠脑缺血和神经元保护作用的实验研究上海中医药杂志2006年第40卷第9期SH.J.TCMSep..2006;Vo1.40No.9夏天无注射液对小鼠脑缺血和神经元保护作用的实验研究余丽梅文国容邓江金风孙安盛黄燮南遵义医学院(贵州遵义563003)张骏遵义医学院附属医院(贵州遵义563003)【摘要】目的观察夏天无注射液对小鼠脑缺血和神经元损伤的保护作用,并探讨其作用机制.方法采用小鼠断头张口喘气模型初步筛选ICDP对动物脑缺血的影响,分光光度法测定脑组织乙酰胆碱酯酶(AchE)和蛋白含量:原代培养乳鼠神经元制备低糖缺氧模型,MqF染色测定神经元细胞活力,流式细胞仪观察神经元凋亡.结果静脉注射ICDP2ms/ks,5ms/ks组后可明显延长小鼠张口喘气时间,且ICDP5ms/ks组断头张口喘气模型小鼠脑组织内AchE活性明显降低.低糖缺氧6h后,ICDP(含生物碱5ng/ml一500ng/m1)明显增加神经元的存活率,并可抑制神经元凋亡.结论ICDP对脑缺血损伤有保护作用,可降低脑组织内AchE活性,保护神经元,抗神经元凋亡可能是其抗脑缺血损伤的机制之一.【关键词】夏天无注射液;小鼠;脑缺血;AchE;神经元;凋亡【中图分类号】R285.5;R743I310.53【文献标识码】A【文章编号】1007—1334(2006)09—0070—03ProtectiveEffectsofInjectionofCorydalisDecumbensPersonCerebrallschemiaandNeuronsYULi—meiWENGuo-rongDENGJiangJINFengSUNAn~hengHUANGXie-nanZunyiMedicalCollegeZHANGJunTheHospitalAffiliatedtoZunyiMedicalCollegeAbstract:ToinvestigateprotectiveeffectofInjectionofCorydalisDecumbensPets(ICDP)oncerebralischemiaandneurons,explorepotentialmechanismofICDP,theeffectofICDPoninjuryofcerebralischemiawaspreliminarilyscreenedindecapitatedgaspingmousemode1.AchEandproteinlevelweredeterminedbyspectrophotometricmethodinbraintissue.Theneuronmodelofanoxiaandglucose-derivedinjurywasmadeinprimaryculturecorticalneuronsofSDrats.TheviabilityofnervecellswasevaluatedbyMqTmethod.Theapoptosisofneuronswasdetectedbyflowcytometry.TheaveragegaspingtimewassignificantlyprolongedinmicetreatedwithICDP2mgkg一and5mgkg～.AchEactivityofICDP5mg?kg一groupwasdecreasedinbraintissueofdecapitatedgaspingmice,ThepercentageofsurvivalceilswasobviouslyincreasedinICDP5ng?ml～,50ng?ml一and500ng?ml～groupsafteranoxiaandglucose—derivedinjury,butthepercentageofneuronsapoptosiswasdecreased.TheresultsshowthatICDPhasprotectiveeffectoninjuryofcerebralischemiapossiblybydecreasingAchEactivity,protectingneuronsandantiapoptosis.KeyWords:InjectionofCorydalisDecumbensPets(ICDP);mice;cerebralischemia;AchE;neuron;apoptosis中药夏天无[Corydalisdecumbens(Thunb.)Pers.]为罂粟科植物伏生紫堇的干燥块茎.具有活血祛瘀作用.夏天无总碱(CoryadlisambailisMigototalalkaloids,COAMTA)能增强小鼠对低压缺氧的耐受力.抑SU--磷酸腺苷(ADP)诱导的大鼠血小板聚