明日叶查尔酮对小鼠急性辐射损伤的防护作用分析-protective effect of chalcone on acute radiation injury in mice.docxVIP

万方数据 万方数据 （5）骨髓嗜多染红细胞微核率：高剂量组和辐射损伤组的骨髓嗜多染红细胞 微核率分别为（8.667±0.543）‰和（18.912±0.525）‰，辐射损伤组较高剂 量组显著性降低（p0.05）。 结论：明日叶查尔酮可抑制辐射损伤引发的小鼠肝脏总抗氧化能力、超氧化物歧 化酶活性的降低和脂质过氧化物水平的升高；上调肝脏细胞 Bcl-2 蛋白和下调 Bax 蛋白的表达水平，提高脾脏细胞的增殖活性，降低骨髓嗜多染红细胞微核率， 对小鼠急性辐射损伤有一定的防护作用。 学生：翟苗苗;卫生毒理学 指导教师：钟进义教授 关键词：查尔酮；辐射损伤；氧化应激；Bcl-2；Bax；增殖活性；微 核率 Research on protective effect of Ashitaba Chalcone on acute radiation-injured mice ABSTRACT Objective: To study the protective effect of Ashitaba Chalcone (AC) on acute radiation-injured mice. Method: Fifty healthy kunming mice with half males and females were randomly divided into 5 groups after feeding in our laborotary for one week, including control group, radiation injury group, low dose of AC group, middle dose of AC group and high dose of AC group, per group consisted of five mice. The above each experiment group was given by gavage once a day for five days with 4mg/kg AC for low dose group, 20 mg/kg AC for middle dose group, 40 mg/kg for high dose group, while the control group and radiation injury group were given saline by gavage. At sixth day, all experiment group were irradiated with 4 Gy x-ray once except blank control group, then all mice were given different dose of AC as usual for the next five days. At the sixth day, all mice were sacrificed to detected the following indicators: Detecting the body weight of mice at the day before gavage, the day before irradiation and the sixth day after irradiation with precision electronic balance. Determination of T-AOC, MDA and SOD of liver by spectrophotometric method. Determination of Bcl-2 and Bax protein expression of liver by Immunohistochemical method. Detecting proliferation activity of spleen cell by MTT method. Determination of the bone marrow polychromatic erythrocyte micronuclei ratio. Results: (1) The body weight of mice exposure to 4 mg/kg AC group was 24.396 ± 1.388 g and 24.792 ± 0.919 g respectively at the day before irradiation and the sixth day after irradiation; 20 mg/kg AC group was 24.375 ± 1.24