模拟co,2气腹对胃癌mkn-45细胞整合素介导的fak信号通路的影响-effects of simulated co, 2 pneumoperitoneum on fak signal pathway mediated by integrin in gastric cancer mkn - 45 cells.docxVIP

模拟co,2气腹对胃癌mkn-45细胞整合素介导的fak信号通路的影响-effects of simulated co, 2 pneumoperitoneum on fak signal pathway mediated by integrin in gastric cancer mkn - 45 cells.docx

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模拟co,2气腹对胃癌mkn-45细胞整合素介导的fak信号通路的影响-effects of simulated co, 2 pneumoperitoneum on fak signal pathway mediated by integrin in gastric cancer mkn - 45 cells

第三军医大学硕士学位论文 第三军医大学硕士学位论文 4 4 hours,4 hours were significantly different from the control(p0.01),while the group of cultured in normal environment for 1 hour after being treated for 4 hours was not significantly different from the control(p0.05); 4. The Integrinβ1 expressions of all groups were not significantly different from each other. Conclusion: In vitro, CO2 pneumoperitoneum can not promote the FAK expression of Gastric Cancer MKN-45 Cell in 4 hours; 5mmHg and 10mmHg CO2 pneumoperitoneum can not effect the FAK’s activity of the Integrin/FAK signal pathway ,but 15mmHg CO2 pneumoperitoneum can activate FAK through increasing FAK Tyr397 phosphorylation, and the activity of FAK is promoted with the time of being treated.Once departs from the CO2 pneumoperitoneum, the activity decreases to the levels that before treated rapidly. CO2 pneumoperitoneum can not promote the Integrinβ1 expression of Gastric Cancer MKN-45 Cell in 4 hours,indicates that CO2 pneumoperitoneum is not through promoting the expressing of Integrinβ1 to activate the FAK of Gastric Cancer MKN-45 Cell. The CO2 pneumoperitoneum pressure used in clinical practice may have no influence on gastric cancer cell proliferation and metastasis through Integrin/FAK signal pathway. KeyWords: CO2 Pneumoperitoneum; Gastric cancer cells; FAK; Phospho-FAK; Integrinβ1 PAGE PAGE 1 英文缩写 英文缩写一览表 英文全称 中文全称 CO2 d carbon dioxide day 二氧化碳 天 FAK Focal Adhesion Kinase 粘着斑激酶 ddH2O double distilled H2O 双蒸水 DMSO dimethylsulfoxide 二甲基亚砜 phospho-FAK Phosphorylated Focal Adhesion Kinase 磷酸化粘着斑激酶 ECM extracellular matrix 细胞外基质 EDTA ethylenediaminetetraacetic acid 乙二胺四乙酸 FCM flowcytometry 流式细胞术 FCS fetal calf serum 胎牛血清 FITC fluorescein isothiocyanate 异硫氰酸荧光素 h hour 小时 kDa kilodalton 千道尔顿 min minute 分钟 ml milliliter 毫升 mmHg millimetre of mercury 毫米汞柱 MTT methyl thiazolyl tetrazolium 四甲基偶氮唑盐 OD opitcal density 光密度值 PBS phosphate buffered solution 磷酸盐缓冲液 rpm round per minute 每分钟转速 s second 秒 Tris trihydroxymethylaminom

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