纳米金粒子对肿瘤细胞辐射敏感性影响的分析-analysis of the effect of nano-gold particles on the radiosensitivity of tumor cells.docxVIP

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2018-07-27 发布于上海
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纳米金粒子对肿瘤细胞辐射敏感性影响的分析-analysis of the effect of nano-gold particles on the radiosensitivity of tumor cells.docx

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纳米金粒子对肿瘤细胞辐射敏感性影响的分析-analysis of the effect of nano-gold particles on the radiosensitivity of tumor cells

PAGE PAGE VI 纳米金粒子对肿瘤细胞辐射敏感性影响的研究中文摘要 (4)浓度在 492-4728μg/ L 范围内，AuNPs、AuNPs@MPA-PEG、AuNPs@PEG 对 Hela 细胞都没有产生显著地辐射增敏作用。关键词：柠檬酸钠；硼氢化钠；氯金酸；纳米金粒子； NH2-PEG-NH2 ； SH-PEG-O-CH3；Hela 细胞；细胞增殖；细胞吸收量；辐射敏感性作者：刘瑞芳指导老师：张保国 Abstract The effect of gold nanoparticles on radiosensitization of Hela cancer cells The effect of gold nanoparticles on radiosensitization of Hela cancer cells Abstract Objective: To explore the efffect of AuNPs ， AuNPs@MPA-PEG and AuNPs@PEG on radiosensitization of human uterine cervix cancer cell line(HeLa). gold nanoparticles(AuNPs) were modified with MPA-PEG or SH-PEG-O-CH3. Hela cells were exposed to different conceration AuNPs ， AuNPs@MPA-PEG or AuNPs@PEG combined with radiation, then the cells survival rate of Hela cells was studied and the intracellular uptake of AuNPs, AuNPs@MPA-PEG and AuNPs@PEG was measured. Methods: Aqueous solutions of AuNPs were prepared by sodium borohydride reduction of tetrachloroauric(III) acid solution in the presence of sodium citrate. Gold nanoparticles(AuNPs) were modified with MPA-PEG or SH-PEG-O-CH3. The UV-Vis spectra of AuNPs，AuNPs@MPA-PEG and AuNPs@PEG were collected with UV-Vis spectrometry. Size and size distribution were measured using dynamic light scattering. Hela cells were cultured in differect concentrations of AuNPs，AuNPs@MPA-PEG and AuNPs@PEG in vitro.The inhibitory ratio of the cells were measured by trypan blue exclusion staining assay. Colon forming assay is carried out to study the cells survival rate of HeLa cells after exposure to AuNPs，AuNPs@MPA-PEG and AuNPs@PEG combined with radiation. The intracellular uptake of AuNPs，AuNPs@MPA-PEG and AuNPs@PEG was determined using inductively-coupled plasma ectrometer. Results: The size of AuNPs prepared by adding the mixture solution of sodium citrate and sodium borohydride to chloroauric acid at 75℃ is small and homogeneous. Compaired with the UV-VIS spectra of AuNPs which was prepared at room temperature，there was a blue