三种侧耳多糖的提取及其抗氧化活性研究-study on extraction and antioxidant activity of polysaccharides from three species of pleurotus ostreatus.docxVIP
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三种侧耳多糖的提取及其抗氧化活性研究-study on extraction and antioxidant activity of polysaccharides from three species of pleurotus ostreatus
三种侧耳多糖的提取及其抗氧化活性分析andP.corncopiaeSS-01wereconcentration-dependentatthedosagerangeof12-60μg/mL.TheinhibitionpercentageofIPSofP.corncopiaeSS-01at60μg/mLwas38.7±3.1%(P0.01),whichwas20.5±1.7%and140.8±12.8%higherthanthatofP.nebrodensisSJ-02(32.2±2.8%,P0.05)andP.eryngiiSI-01(16.1±1.5%,P0.01),respectively.TheinhibitionpercentagesofIPSofP.corncopiaeSS-01,P.nebrodensisSJ-02andP.eryngiiSI-01at60μg/mLwere20.3±1.8%(P0.01),19.1±1.5%(P0.01),and16.3±1.3%(P0.05),respectively,andtheDPPHscavengingrateofIPSofP.corncopiae(SS-01)at60μg/mLwas20.5±1.7%(P0.01),whichwas14.5±1.2%and21.4±1.7%higherthanthatofP.nebrodensisSJ-02(17.9±1.6%,P0.05)andP.eryngiiSI-01(16.8±1.4%,P0.05),respectively.EPSofP.eryngiiSI-01alsohadsignificantlyscavengingeffectsonsuperoxideanion,hydroxylandDPPHradicalsandreducingpower.Thescavengingrateofsuperoxideanion,hydroxylradicalsandDPPHofEPSofP.eryngiiSI-01reached(38.69±2.59%,P0.01),(59.63±3.72%,P0.01)and(66.36±4.42%,P0.05)atadosageof400mg/L,respectively.TheinvivoantioxidanteffectsofIPSandEPSonsuperoxidedismutase(SOD)andglutathioneperoxidsade(GSH-Px)activityandmalondialdehyde(MDA)contentintheblood,heart,liver,kidney,spleenofmicewereinvestigate.ResultsshowedthatIPSandEPScouldremarkablyincreasetheactivityofSODandGSH-Px,decreasetheMDAcontent.TheresultssuggestthattheIPSandEPSofPleurotussp.hadsignificantlyantioxidantactivityinvivoandinvitro,whichcouldbeusedasnaturalandpotentialantioxidants.Keywords:Pleurotussp.;Intracellularpolysaccharide;Exopolysaccharide;Antioxidation符号说明BHT:2,6-Di-tert-butyl-4-methylphenol,2,6-二叔丁基对甲酚DPPH:2,2-diphenyl-1-picrylhydrazyl,二苯基苦基苯肼自由基DTNB:5,5-dithiobis-(2-nitrobenzoicacid),5,5-二硫硝基苯甲酸EPS:extracellulapolysaccharides,胞外多糖EPS-1:extracellulapolysaccharides,去杂质后的EPSGSH:Glutathione,谷胱甘肽GSH-Px:Glutathioneperoxidsade,谷胱甘肽过氧化物酶IPS:intracellularpolysaccharide,胞内多糖IPS-1:intracellularpolysaccharide,去杂质后的IPSMDA:Malondialdehyde,丙二醛NBT:Nitrotetrazoliumbluechloride,氮蓝四唑PB:Plackett-Burmandesign,PB设计RSM:responsesurfacemethodology,响应面法SOD:Superoxidedismutase,超氧化物歧
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