三株滨海来源植物内生真菌次级代谢产物及其活性研究-secondary metabolites and activities of three endophytic fungi from coastal plants.docx

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2018-07-30 发布于上海
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三株滨海来源植物内生真菌次级代谢产物及其活性研究-secondary metabolites and activities of three endophytic fungi from coastal plants.docx

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三株滨海来源植物内生真菌次级代谢产物及其活性研究-secondary metabolites and activities of three endophytic fungi from coastal plants

阐明了全部化合物的结构，新化合物5个，新天然产物1个，并对部分系列化合物进行了抗肿瘤活性和抗菌活性的评价。本研究进一步丰富了海洋天然产物化学化合物库；发现了具有新的抗肿瘤细胞活性的化合物，为抗肿瘤天然产物的研究奠定了基础。关键词：植物内生真菌；次级代谢产物；抗肿瘤活性SecondaryMetabolitesandTheirActivitiesofThreeEndophyticFungifromCoastalAreaAbstractThereweremorethanonemillionendophyticfungiinnature.Becauseofthedifferentkindsofplantsordifferentlivingenvironments,thesecondarymetabolitesofendophyticfungiwereextremelydiversity.Thestatisticalanalysesshowedthat51%ofthesecondarymetabolitesobtainedfromendophyteshadbiologicallyactivity,indicatingthatendophytesareimportantpotentialsourcesofexploitationfornewnaturalproducts.Inrecentyears,endophytesarepaidmoreandmoreattentions,especiallylivinginunusualorspecializedecologicalnichessuchasocean,saltern,alkaline,endophytes,etc,becauseofoccurringspecialphysiochemicalandbiologicalprocesses.245endophyticfungiobtainedfrom17plantslivingintheareaalongthecoastofLaizhouBayinDongying,China.Byantitumorbioassayscreening,27activestrainswerechosen.CombinedwithTLCandHPLC,chemicalanalyseswereperformedfortheactivesecondarycompoundsofthese27strains,and3fungiofSXH-65(Penicilliumsp.SXH-65),SXH-63(Penicilliumchrysogenum)andSXH-69(Emericellanidulans)wereselectedforfurtherstudy.Applyingvariousofextractandchromatographymethods,35compoundsincluding21(3ofthemarenewcompounds,1ofthemisnewnaturalproduct)compoundsisolatedfromtheorganicextractofSXH-65,4compounds(2ofthemarenewcompounds)isolatedfromthestrainSXH-63,and10compoundsisolatedfromthestrainSXH-69,wereobtained.Thestructuretypeincludedmeroterpenoids,benzodiazepinealkaloids,pyrones,xanthones,benzenederivations,andsoon.Thecytotoxicitiesofmeroterpenoids,pyronesandbenzodiazepinealkaloidswereevaluatedagainstHela,HL-60,A549andK562celllinesandexhibitingweakcytotoxicitieswithIC50valuesrangedfrom24to60μM.AndthebenzodiazepinealkaloidshadnotantimicrobialactivityagainstProteusbacillus,Escherichiacoli,Bacillussubtillis,Micrococcustetragenusat600μg/mL.Thisisthefirstreportfortheircytotoxitiesandantimicrobialactivity.Furtherstudyshouldbeconductedtotestt